概述

  • 产品名称Anti-cIAP1抗体
    参阅全部 cIAP1 一抗
  • 描述
    兔多克隆抗体to cIAP1
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    The immunogen sequence of Rabbit polyclonal to cIAP1 (ab2399) was a peptide sequence flanking amino acid 530 of human cIAP1. (Peptide available as ab38272.)

  • 阳性对照
    • WB: Jurkat cell lysate, PC12 cell lysate, rat kidney tissue lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab2399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 69 kDa).Can be blocked with cIAP1 peptide (ab38272).

靶标

  • 功能Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
  • 组织特异性Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.
  • 序列相似性Belongs to the IAP family.
    Contains 3 BIR repeats.
    Contains 1 CARD domain.
    Contains 1 RING-type zinc finger.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • API 1 antibody
    • API1 antibody
    • Apoptosis inhibitor 1 antibody
    • Baculoviral IAP repeat containing 2 antibody
    • Baculoviral IAP repeat containing protein 2 antibody
    • Baculoviral IAP repeat-containing protein 2 antibody
    • BIRC 2 antibody
    • BIRC2 antibody
    • BIRC2_HUMAN antibody
    • C IAP1 antibody
    • C-IAP1 antibody
    • cIAP 1 antibody
    • cIAP1 antibody
    • HIAP 2 antibody
    • HIAP-2 antibody
    • HIAP2 antibody
    • IAP 2 antibody
    • IAP homolog B antibody
    • IAP-2 antibody
    • IAP2 antibody
    • Inhibitor of apoptosis protein 2 antibody
    • MIHB antibody
    • NFR2 TRAF signalling complex protein antibody
    • RING finger protein 48 antibody
    • RNF 48 antibody
    • RNF48 antibody
    • TNFR2 TRAF signaling complex protein 2 antibody
    • TNFR2-TRAF-signaling complex protein 2 antibody
    see all

Anti-cIAP1 antibody 图像

  • Anti-cIAP1 antibody (ab2399) + Jurkat Cell lysate

    Predicted band size : 69 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab2399 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2399, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-cIAP1 antibody (ab2399)参考文献

This product has been referenced in:
  • Yi JM  et al. Dual targeting of microtubule and topoisomerase II by a-carboline derivative YCH337 for tumor proliferation and growth inhibition. Oncotarget 6:8960-73 (2015). Human . Read more (PubMed: 25840421) »
  • Ghadimi MP  et al. Survivin is a viable target for the treatment of malignant peripheral nerve sheath tumors. Clin Cancer Res 18:2545-57 (2012). WB ; Human . Read more (PubMed: 22407831) »

See all 7 Publications for this product

Product Wall

Thank you for your email.

The peptide is from the sequence around amino acid 530 of human cIAP1. This sequence does not match with the immunogen sequence of polyclonal and monoclonal antibodies you have so this peptide ab38272 would be of no u...

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Thank you for contacting us.

Could you please explain what do you mean by other antibodies? The antibodies against the same peptide should be same however difference can be seen in specificity, purity etc.

The peptide ab38272 is from ...

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Thank you for your phone call. I am very sorry to hear that these 3 antibodies are not providing satisfactory results. The details provided will enable us to investigate these 3 cases and will provide us with vital information for monitoring product...

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Thank you for your inquiry. We source this antibody from an outside laboratory, and unfortunately we do not have access to the exact sequence used for the immunogen. If you plan to use the antibody on a fragment of the protein, we may be able t...

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Thank you for your enquiry. The immunogen sequence of Rabbit polyclonal to cIAP1 (ab2399) was a peptide sequence flanking amino acid 530 of human cIAP1. I hope this information helps, please do not hesitate to contact us if you need any more ...

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Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"