概述

  • 产品名称Anti-CIAO1抗体
    参阅全部 CIAO1 一抗
  • 描述
    兔多克隆抗体to CIAO1
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 143-192 (SVLNSHTQDV KHVVWHPSQE LLASASYDDT VKLYREEEDD WVCCATLEGH) of human CIA01 (NP_004795).

  • 阳性对照
    • 721_B cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified by peptide affinity chromatography method.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

IHC-P 1/100.

靶标

Anti-CIAO1 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling CIAO1 with ab83088 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of alveolar type I cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - CIAO1. Right - Merge.
  • Anti-CIAO1 antibody (ab83088) at 1 µg/ml + 712_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 26 kDa,64 kDa. We are unsure as to the identity of these extra bands.


  • Predicted band size : 38 kDa
    Western Blot using ab83088 at 1.0ug/ml. Sample type: Human Hela cell Lysate


  • Predicted band size : 38 kDa
    Western Blot using ab83088 at 1.0ug/ml. Sample type: Human MCF7 cell Lysate

Anti-CIAO1 antibody (ab83088)参考文献

ab83088 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab83088.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"