Anti-CHTF18抗体[2182C3a] (ab51331)


  • 产品名称Anti-CHTF18抗体[2182C3a]
    参阅全部 CHTF18 一抗
  • 描述
    小鼠单克隆抗体[2182C3a] to CHTF18
  • 经测试应用适用于: WB, IP, Dot blotmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human CHTF18 fragment

  • 阳性对照
    • HT1080 whole cell lysate.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, 8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号2182C3a
  • 同种型IgG1
  • 研究领域


Our Abpromise guarantee covers the use of ab51331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot
  • 应用说明Dot: Use at an assay dependent dilution.
    IP: Use at 10µg/mg RIPA lysate of HeLa cells.
    WB: 1/100. Detects a band of approximately 118 kDa (predicted molecular weight: 107 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). The CTF18-RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. It also interacts with and stimulates DNA polymerase POLH.
    • 序列相似性Belongs to the activator 1 small subunits family. CTF18 subfamily.
    • 细胞定位Nucleus. Associates with chromatin during S phase.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 6030457M03Rik antibody
      • C16orf41 antibody
      • C321D2.2 antibody
      • C321D2.3 antibody
      • C321D2.4 antibody
      • CHL12 antibody
      • chromosome transmission fidelity factor 18 homolog (S. cerevisiae) antibody
      • Chromosome transmission fidelity protein 18 homolog antibody
      • chtf18 antibody
      • Ctf18 antibody
      • CTF18_HUMAN antibody
      • hCTF18 antibody
      • MGC38008 antibody
      • RUVBL antibody
      see all

    Anti-CHTF18 antibody [2182C3a] 图像

    • Anti-CHTF18 antibody [2182C3a] (ab51331) at 1/100 dilution + HT1080 whole cell lysate at 25 µg

      anti-mouse IgG at 1/2500 dilution
      Developed using the ECL technique

      Predicted band size : 107 kDa
      Observed band size : 118 kDa (why is the actual band size different from the predicted?)
    • Immunoprecipitation analysis using ab51331 at 5ug/500ug RIPA lysate of HeLa cells. Immunoblot using ab51331 at 1/100 dilution and anti-mouse IgG at 1/2500 dilution as secondary.

    Anti-CHTF18 antibody [2182C3a] (ab51331)参考文献

    ab51331 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Male Meiotic Cells)
    Specification Male Meiotic Cells
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step BSA and Fish Gelatin as blocking agent for 45 minute(s) · Concentration: 0.2% · Temperature: 24°C

    Abcam user community

    Verified customer

    提交于 Jul 06 2012

    I am very pleased to hear you would like to accept our offer and test ab51331 in mouse.

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    This code will give you: 1 free primary antibody or value off the order before the expira...

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    Thank you for your reply.

    The immmunogen for ab51331 CHTF18 antibody [2182C3a] is as follows:

    As the alignment is re...

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    Thank you for your enquiry.

    I am sorry to confirm that as far as we are aware, ab51331 CHTF18 antibody [2182C3a] has never been tested with samples from mouse. All tested and guaranteed species cross-reactivity information is stated on our d...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HCT116 (colorectal))
    Specification HCT116 (colorectal)
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton X100 in PBS

    Dr. Kirk Mcmanus

    Verified customer

    提交于 Jan 12 2009