Anti-Chromogranin C/SGII抗体[SGII 6B1/3] (ab20245)
Key features and details
- Mouse monoclonal [SGII 6B1/3] to Chromogranin C/SGII
- Suitable for: ICC/IF, IHC-P
- Reacts with: Rat
- Isotype: IgG2b
概述
-
产品名称
Anti-Chromogranin C/SGII抗体[SGII 6B1/3]
参阅全部 Chromogranin C/SGII 一抗 -
描述
小鼠单克隆抗体[SGII 6B1/3] to Chromogranin C/SGII -
宿主
Mouse -
特异性
SGII6B1/3 has potential uses for chromatogranin diagnostics. -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide corresponding to Rat Chromogranin C/SGII (C terminal).
Sequence:KEENSRENPF
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
-
纯度
Protein A/G purified -
克隆
单克隆 -
克隆编号
SGII 6B1/3 -
骨髓瘤
Sp2 -
同种型
IgG2b -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab20245于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (1) |
Use at an assay dependent concentration.
|
IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Secretogranin-2 is a neuroendocrine secretory granule protein, which is the precursor for biologically active peptides. -
序列相似性
Belongs to the chromogranin/secretogranin protein family. -
细胞定位
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
-
数据库链接
- Entrez Gene: 24765 Rat
- SwissProt: P10362 Rat
- Unigene: 45602 Rat
-
别名
- CHGC antibody
- Chromogranin C antibody
- Chromogranin-C antibody
see all
图片
-
Confocal image of immunofluorescence staining with Mouse monoclonal [SGII 6B1/3] to Chromogranin C/SGII;(ab20245; 2µg/ml for 1h at room temperature) in red and nuclear counterstain in blue on Rat Cell (Hippocampal neuron culture). Cells were fixed with paraformaldehyde; BSA (5%) was used as a blocking agent (40 minutes at room temperature). Secondary antibody was Goat anti-mouse IgG2b Alexa Fluor 568 Conjugation (1/600).
-
IHC image of ab20245 staining in normal rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab20245, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (7)
ab20245 被引用在 7 文献中.
- Çakmakli GY et al. Striatal Neurotransmitter Release-related Presynaptic Proteins in L-dopa Induced Dyskinesia in a Model of Parkinsonism. Noro Psikiyatr Ars 55:73-79 (2018). PubMed: 30042645
- Chen Y et al. An efficient two-step subcellular fractionation method for the enrichment of insulin granules from INS-1 cells. Biophys Rep 1:34-40 (2015). PubMed: 26942217
- Berard AR et al. Differential Reovirus-Specific and Herpesvirus-Specific Activator Protein 1 Activation of Secretogranin II Leads to Altered Virus Secretion. J Virol 89:11954-64 (2015). PubMed: 26378181
- Oh JY et al. Identification of the HSPB4/TLR2/NF-?B axis in macrophage as a therapeutic target for sterile inflammation of the cornea. EMBO Mol Med : (2012). ICC/IF ; Rat . PubMed: 22359280
- Kim CH et al. Norepinephrine deficiency is caused by combined abnormal mRNA processing and defective protein trafficking of dopamine beta-hydroxylase. J Biol Chem 286:9196-204 (2011). WB . PubMed: 21209083
- Urbé S et al. Homotypic fusion of immature secretory granules during maturation in a cell-free assay. J Cell Biol 143:1831-44 (1998). PubMed: 9864358
- Dittié AS & Tooze SA Characterization of the endopeptidase PC2 activity towards secretogranin II in stably transfected PC12 cells. Biochem J 310 ( Pt 3):777-87 (1995). PubMed: 7575410