概述

  • 产品名称Anti-Chromogranin C抗体
    参阅全部 Chromogranin C 一抗
  • 描述
    兔多克隆抗体to Chromogranin C
  • 经测试应用适用于: ICC/IF, WB, IP, IHC-FoFr, IHC-Fr, ICCmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to Ovalbumin, corresponding to N terminal amino acids 1-19 of Chromogranin C

  • 常规说明Dilute in PBS or medium which is identical to that used in the assay system.

性能

应用

Our Abpromise guarantee covers the use of ab12241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 17933551
WB 1/250 - 1/500. Predicted molecular weight: 74 kDa.
IP 1/25 - 1/100.
IHC-FoFr Use at an assay dependent concentration.
IHC-Fr 1/100.
ICC Use at an assay dependent concentration.

靶标

Anti-Chromogranin C antibody 图像

  • ab12241 staining Chromogranin C in Mouse primary cortical neuronal cells treated with IL-1β by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.2% Triton X-100 for 15 minutes and blocked with 10% serum for 1 hours at room temperature°C. Samples were incubated with primary antibody (1/1000 in 0.3% BSA) for 1 hour at room temperature. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/2000) was used as the secondary antibody.

Anti-Chromogranin C antibody (ab12241)参考文献

This product has been referenced in:
  • Blanco EH  et al. An amphipathic alpha-helix in the prodomain of cocaine and amphetamine regulated transcript peptide precursor serves as its sorting signal to the regulated secretory pathway. PLoS One 8:e59695 (2013). ICC/IF ; Rat . Read more (PubMed: 23527253) »
  • Kuczewski N  et al. Backpropagating action potentials trigger dendritic release of BDNF during spontaneous network activity. J Neurosci 28:7013-23 (2008). ICC/IF ; Rat . Read more (PubMed: 18596175) »

See all 4 Publications for this product

Product Wall

This antibody has been tested on frozen sections of human pituitary. We do not know if it will work on formalin-fixed, paraffin-embedded tissue sections. Ab12241 has also been tested on monolayer cultures of a pituitary tumor cell line, GH4C1, for ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"