Anti-Chromogranin B抗体(ab85286)

概述

  • 产品名称
    Anti-Chromogranin B抗体
    参阅全部 Chromogranin B 一抗
  • 描述
    兔多克隆抗体to Chromogranin B
  • 经测试应用
    适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human Chromogranin B.

  • 阳性对照
    • WB: Human adrenal gland tissue lysate and PC12 (untreated and NGF treated) whole cell lysates. IHC-P: Rat normal brain and human normal pancreas tissues. ICC/IF: PC12 cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab85286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 78 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能
    Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.
  • 组织特异性
    Expressed in the adrenal medulla, and in pheochromocytoma. Not expressed in liver.
  • 序列相似性
    Belongs to the chromogranin/secretogranin protein family.
  • 翻译后修饰
    Extensively processed by limited proteolysis at conserved basic residues. Alternative processing are seen in different tissues.
  • 细胞定位
    Secreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCB peptide antibody
    • Cg B antibody
    • CgB antibody
    • Chgb antibody
    • Chromogranin-B antibody
    • ChromograninB antibody
    • SCG1 antibody
    • SCG1_HUMAN antibody
    • Secretogranin 1 antibody
    • Secretogranin B antibody
    • Secretogranin I antibody
    • SgI antibody
    see all

图片

  • All lanes : Anti-Chromogranin B antibody (ab85286) at 1 µg/ml

    Lane 1 : Human adrenal normal tissue lysate - total protein (ab29249)
    Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) NGF-Treated Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute
  • IHC image of Chromogranin B staining in Rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85286, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab85286 stained PC12 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85286, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

ab85286 has not yet been referenced specifically in any publications.

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