Anti-Chromogranin B抗体(ab12242)

概述

  • 产品名称Anti-Chromogranin B抗体
    参阅全部 Chromogranin B 一抗
  • 描述
    兔多克隆抗体to Chromogranin B
  • 特异性Ab12242 recognises chromogranin B.
  • 经测试应用适用于: WB, ICC/IF, IHC-Fr, ICC, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    C-terminal 15 amino acids of chromogranin B conjugated to ovalbumin.

性能

应用

Our Abpromise guarantee covers the use of ab12242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 78.2 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 24454876
IHC-Fr 1/100 - 1/500.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.
  • 组织特异性Expressed in the adrenal medulla, and in pheochromocytoma. Not expressed in liver.
  • 序列相似性Belongs to the chromogranin/secretogranin protein family.
  • 翻译后修饰Extensively processed by limited proteolysis at conserved basic residues. Alternative processing are seen in different tissues.
  • 细胞定位Secreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCB peptide antibody
    • Cg B antibody
    • CgB antibody
    • Chgb antibody
    • Chromogranin-B antibody
    • ChromograninB antibody
    • SCG1 antibody
    • SCG1_HUMAN antibody
    • Secretogranin 1 antibody
    • Secretogranin B antibody
    • Secretogranin I antibody
    • SgI antibody
    see all

Anti-Chromogranin B antibody 图像

  • ab12242(1:1000) staining Chromogranin B in human pancreas (IgG inset) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of Chromogranin B in secretory compartments of the pancreas.
    Insert panel depicts negative control (no primary antibody).
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX.
    Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.<

Anti-Chromogranin B antibody (ab12242)参考文献

This product has been referenced in:
  • Wang H  et al. RanBP9 overexpression accelerates loss of pre and postsynaptic proteins in the AP?E9 transgenic mouse brain. PLoS One 9:e85484 (2014). WB, ICC/IF ; Mouse . Read more (PubMed: 24454876) »
  • Weisbrod AB  et al. Altered PTEN, ATRX, CHGA, CHGB, and TP53 expression are associated with aggressive VHL-associated pancreatic neuroendocrine tumors. Horm Cancer 4:165-75 (2013). IHC-P ; Human . Read more (PubMed: 23361940) »

See all 5 Publications for this product

Product Wall

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Thank you for your email. We unfortunately do not have ab8205 anymore and we also do not have exactly same product in catalogue.  However we do have many "Chromogranin A" antibodies that are very well characterized. You may consider trying.  Th...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - other (Cerebrospinal fluid (CSF))
Loading amount 3 µg
Specification Cerebrospinal fluid (CSF)
Gel Running Conditions Non-reduced Denaturing (12% SDS)
Blocking step Milk as blocking agent for 17 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Ms. Christiane Rewerts

Verified customer

提交于 May 29 2009

Thank you for your enquiry and your interest in our products. Here is the protocol that was used to develop this antibody: Frozen pellets of PC12 pheochromocytoma cells were thawed (1 g wet weight/10 ml) in a buffer containing 20 mM Tris-HCl (pH ...

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This product is sold as whole antiserum, not as purified antibody. In general, we estimate that the total Ig concentration in serum is approx. 10 mg/ml, and the specific antibody component is approx. 10% of this. I hope this helps.

Thank you for your enquiry. This product is whole, undiluted antiserum, so it is not in a storage buffer. To dilute for use, we recommend either PBS or a buffer/medium that is compatible with the user's assay system.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"