RabMAb

Anti-Chromogranin A抗体[EP1031Y] (ab52983)

概述

  • 产品名称Anti-Chromogranin A抗体[EP1031Y]
    参阅全部 Chromogranin A 一抗
  • 描述
    兔单克隆抗体[EP1031Y] to Chromogranin A
  • 经测试应用适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-terminus of human Chromogranin A was used as an immunogen.

  • 阳性对照
    • PC12 cytoplasmic cell lysate Human pancreatic tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab52983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1e+006. Detects a band of approximately 80-90 kDa (predicted molecular weight: 51 kDa).Can be blocked with Chromogranin A peptide (ab138920).
IP 1/30.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for Flow Cyt or ICC.
  • 靶标

    • 功能Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
    • 序列相似性Belongs to the chromogranin/secretogranin protein family.
    • 翻译后修饰Sulfated on tyrosine residues and/or contains sulfated glycans.
      O-glycosylated with core 1 or possibly core 8 glycans.
    • 细胞定位Secreted. Neuroendocrine and endocrine secretory granules.
    • Information by UniProt
    • 数据库链接
    • 形式According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
    • 别名
      • beta Granin antibody
      • betagranin (N-terminal fragment of chromogranin A) antibody
      • catestatin antibody
      • CgA antibody
      • CHG A antibody
      • Chga antibody
      • chromofungin antibody
      • Chromogranin A parathyroid secretory protein 1 antibody
      • Chromogranin A precursor antibody
      • ChromograninA antibody
      • CMGA_HUMAN antibody
      • ER-37 antibody
      • Pancreastatin antibody
      • Parastatin antibody
      • Parathyroid secretory protein 1 antibody
      • Pituitary secretory protein I antibody
      • Secretory protein I antibody
      • SP I antibody
      • SP-I antibody
      • SP1 antibody
      • SPI antibody
      • Vasostatin antibody
      • Vasostatin I antibody
      • Vasostatin II antibody
      see all

    Anti-Chromogranin A antibody [EP1031Y] 图像

    • Anti-Chromogranin A antibody [EP1031Y] (ab52983) at 1/1000000 dilution + PC12 cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP labelled at 1/2000 dilution

      Predicted band size : 51 kDa
      Observed band size : 80-90 kDa (why is the actual band size different from the predicted?)
    • ab52983, at a 1/250 dilution, staining Human Chromogranin A in Pancreas using Immunohistochemistry, Paraffin Emebedded tissue.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Chromogranin A antibody [EP1031Y] (ab52983)参考文献

    ab52983 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.

    As requested, the blocking peptide for the Chromogranin A antibody ab52983 has been added to our catalogue. The catalogue reference number is ab138920 and the link to the datasheet is http://www.abcam.com/ab138920....

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"