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RabMAb

Anti-Chromogranin A抗体[EP1031Y] (ab52983)

概述

  • 产品名称
    Anti-Chromogranin A抗体[EP1031Y]
    参阅全部 Chromogranin A 一抗
  • 描述
    兔单克隆抗体[EP1031Y] to Chromogranin A
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-terminus of human Chromogranin A was used as an immunogen.

  • 阳性对照
    • PC12 cytoplasmic cell lysate Human pancreatic tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    A trial size is available to purchase for this antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab52983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1e+006. Detects a band of approximately 80-90 kDa (predicted molecular weight: 51 kDa).Can be blocked with Chromogranin A peptide (ab138920).
IP 1/30.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 应用说明
    Is unsuitable for Flow Cyt or ICC.
  • 靶标

    • 功能
      Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
    • 序列相似性
      Belongs to the chromogranin/secretogranin protein family.
    • 翻译后修饰
      Sulfated on tyrosine residues and/or contains sulfated glycans.
      O-glycosylated with core 1 or possibly core 8 glycans.
    • 细胞定位
      Secreted. Neuroendocrine and endocrine secretory granules.
    • Information by UniProt
    • 数据库链接
    • 形式
      According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
    • 别名
      • beta Granin antibody
      • betagranin (N-terminal fragment of chromogranin A) antibody
      • catestatin antibody
      • CgA antibody
      • CHG A antibody
      • Chga antibody
      • chromofungin antibody
      • Chromogranin A antibody
      • Chromogranin A parathyroid secretory protein 1 antibody
      • Chromogranin A precursor antibody
      • ChromograninA antibody
      • CMGA_HUMAN antibody
      • ER-37 antibody
      • Pancreastatin antibody
      • Parastatin antibody
      • Parathyroid secretory protein 1 antibody
      • Pituitary secretory protein I antibody
      • Secretory protein I antibody
      • SP I antibody
      • SP-I antibody
      • SP1 antibody
      • SPI antibody
      • Vasostatin antibody
      • Vasostatin I antibody
      • Vasostatin II antibody
      see all

    图片

    • Anti-Chromogranin A antibody [EP1031Y] (ab52983) at 1/1000000 dilution + PC12 cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP labelled at 1/2000 dilution

      Predicted band size : 51 kDa
      Observed band size : 80-90 kDa (why is the actual band size different from the predicted?)
    • ab52983, at a 1/250 dilution, staining Human Chromogranin A in Pancreas using Immunohistochemistry, Paraffin Emebedded tissue.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    文献

    This product has been referenced in:
    • Simpson PD  et al. Striking Oxygen Sensitivity of the Peptidylglycine a-Amidating Monooxygenase (PAM) in Neuroendocrine Cells. J Biol Chem 290:24891-901 (2015). WB ; Human . Read more (PubMed: 26296884) »

    See 1 Publication for this product

    客户评价及客户问答

    Thank you for contacting us.

    As requested, the blocking peptide for the Chromogranin A antibody ab52983 has been added to our catalogue. The catalogue reference number is ab138920 and the link to the datasheet is http://www.abcam.com/ab138920....

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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