Anti-Chromogranin A抗体(ab85554)
Key features and details
- Rabbit polyclonal to Chromogranin A
- Suitable for: IHC-P, WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Chromogranin A抗体
参阅全部 Chromogranin A 一抗 -
描述
兔多克隆抗体to Chromogranin A -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IP, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Horse, Cow, Dog, Pig, Monkey -
免疫原
-
阳性对照
- Recombinant Human Chromogranin A protein (ab85486) can be used as a positive control in WB. This antibody gave a positive signal in human pancreas tissue lysate. This antibody gave a positive result when used in the following formaldehyde/methanol fixed cell line:PANC-1. ICC/IF: SHSY5Y cells
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85554于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (4) |
Use a concentration of 5 µg/ml.
|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
|
|
IP |
Use a concentration of 5 µg/ml.
|
|
ICC/IF |
Use a concentration of 1 - 5 µg/ml.
|
说明 |
---|
IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
IP
Use a concentration of 5 µg/ml. |
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
靶标
-
功能
Pancreastatin strongly inhibits glucose induced insulin release from the pancreas. -
序列相似性
Belongs to the chromogranin/secretogranin protein family. -
翻译后修饰
Sulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans. -
细胞定位
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
-
数据库链接
- Entrez Gene: 281070 Cow
- Entrez Gene: 100033828 Horse
- Entrez Gene: 1113 Human
- Entrez Gene: 12652 Mouse
- Entrez Gene: 397540 Pig
- Entrez Gene: 24258 Rat
- Omim: 118910 Human
- SwissProt: P05059 Cow
see all -
形式
According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa. -
别名
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
see all
图片
-
ab85554 staining Chromogranin-A precursor in SHSY5Y cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab85554 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
-
IHC image of Chromogranin A staining in Human Pancreas Adenocarcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85554, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
-
Chromogranin A was immunoprecipitated using 0.5mg Mouse Pancreas tissue lysate, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Pancreas tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab85554.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 50kDa; Chromogranin A
-
Anti-Chromogranin A antibody (ab85554) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
IHC image of Chromogranin A staining in Monkey Pancreas sections, performed The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6). Sections were subjected to 2% H202O then Avidin-Biotin to block endogenous peroxidases/biotin. The section was then incubated with ab85554, 1/5000, for 2 hours at 12°C. The section was then counterstained with haematoxylin
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (7)
ab85554 被引用在 7 文献中.
- Reehorst CM et al. EHF is essential for epidermal and colonic epithelial homeostasis, and suppresses Apc-initiated colonic tumorigenesis. Development 148:N/A (2021). PubMed: 34180969
- Sugisawa E et al. RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis. Cell 182:609-624.e21 (2020). PubMed: 32640190
- Dávalos-Salas M et al. Deletion of intestinal Hdac3 remodels the lipidome of enterocytes and protects mice from diet-induced obesity. Nat Commun 10:5291 (2019). PubMed: 31757939
- Bergeron V et al. Deletion of Protein Kinase D1 in Pancreatic ß-Cells Impairs Insulin Secretion in High-Fat Diet-Fed Mice. Diabetes 67:71-77 (2018). PubMed: 29038309
- Moullé VS et al. Glucose and fatty acids synergistically and reversibly promote beta cell proliferation in rats. Diabetologia 60:879-888 (2017). PubMed: 28078385
- Hayase E et al. R-Spondin1 expands Paneth cells and prevents dysbiosis induced by graft-versus-host disease. J Exp Med 214:3507-3518 (2017). PubMed: 29066578
- Terry NA et al. Impaired enteroendocrine development in intestinal-specific Islet1 mouse mutants causes impaired glucose homeostasis. Am J Physiol Gastrointest Liver Physiol 307:G979-91 (2014). PubMed: 25214396