Anti-Chromogranin A抗体(ab15160)

概述

  • 产品名称
    Anti-Chromogranin A抗体
    参阅全部 Chromogranin A 一抗
  • 描述
    兔多克隆抗体to Chromogranin A
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human, Monkey
  • 免疫原

    Recombinant fragment from the C-terminal (Human)

  • 阳性对照
    • Pancreas

性能

应用

Our Abpromise guarantee covers the use of ab15160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 21383188
WB 1/100.
ICC/IF 1/100.
IHC-P 1/400.

靶标

  • 功能
    Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
  • 序列相似性
    Belongs to the chromogranin/secretogranin protein family.
  • 翻译后修饰
    Sulfated on tyrosine residues and/or contains sulfated glycans.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • 细胞定位
    Secreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt
  • 数据库链接
  • 形式
    According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
  • 别名
    • beta Granin antibody
    • betagranin (N-terminal fragment of chromogranin A) antibody
    • catestatin antibody
    • CgA antibody
    • CHG A antibody
    • Chga antibody
    • chromofungin antibody
    • Chromogranin A antibody
    • Chromogranin A parathyroid secretory protein 1 antibody
    • Chromogranin A precursor antibody
    • ChromograninA antibody
    • CMGA_HUMAN antibody
    • ER-37 antibody
    • Pancreastatin antibody
    • Parastatin antibody
    • Parathyroid secretory protein 1 antibody
    • Pituitary secretory protein I antibody
    • Secretory protein I antibody
    • SP I antibody
    • SP-I antibody
    • SP1 antibody
    • SPI antibody
    • Vasostatin antibody
    • Vasostatin I antibody
    • Vasostatin II antibody
    see all

图片

  • ab15160 at 1/250 staining human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed before incubation with the antibody for 30 minutes at 25°C. An HRP conjugated rabbit polyclonal was used as the secondary.

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  • ab15160 staining Chromogranin A in mouse gastric gland cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton ×100 and blocking with 10% serum for 45min at 200C was performed. Samples were incubated with primary antibody (1/100) for 18 hours at 4°C. A Texas Red ®-conjugated goat polyclonal to rabbit IgG was used at dilution 1/200 as secondary antibody.

    See Abreview

  • ab15160 (1/100) staining Chromogranin A in paraffin-embbeded mouse pancreas tissue sections. Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in citrate buffer pH 6.0 and blocking (10 minutes, 1.5% serum). An HRP-conjugated goat polyclonal was used as the secondary. For further experimental details please refer to abreview.

    See Abreview

  • ab15160 staining Chromogranin A in mouse colon tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formalin fixation before heat mediated antigen retrieval with 10mM citric acid buffer and then the sample was blocked for 45 minutes at 20°C. The primary antibody (1/100) was incubated with sample in 10% serum for 18 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution 1/200 as secondary antibody.

    See Abreview

  • Chromogranin A is easily fragmented and both bands observed in the Western Blot above are believed to correspond to Chromogranin A.

文献

This product has been referenced in:
  • Martinez-Valbuena I  et al. Interaction of amyloidogenic proteins in pancreatic ß cells from subjects with synucleinopathies. Acta Neuropathol N/A:N/A (2018). IHC-P ; Human . Read more (PubMed: 29536165) »
  • Norum JH  et al. The tankyrase inhibitor G007-LK inhibits small intestine LGR5+stem cell proliferation without altering tissue morphology. Biol Res 51:3 (2018). IHC-P ; Mouse . Read more (PubMed: 29316982) »

See all 69 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (Colonic entrochromaffin cells)
Permeabilization
No
Gating Strategy
EpCAM+CD45-Chga+
Specification
Colonic entrochromaffin cells
Preparation
Cell harvesting/tissue preparation method: colon dissected and mesentery removed. Feces flushed with PBS. Colon epithelium dissociated and enriched by moderate shaking in 2mM EDTA at 37°C for 15min. Supernatant recovered and washed twice in MACS buffer (PBS supplemented with 5% FBS and 2mM EDTA) for 10min, 300g. Pellet resuspended in 4ml of RPMI (supplemented with 2% FBS and Diapase II at final concentration of 1mg/ml and DNase at final concentration of 10ug/ml) and kept in 37°C water bath for 10min. Cells washed twice with MACS as before.
Sample buffer: PBS supplemented with 5% FBS and 2mM EDTA
Fixation
Acetone
Username

Abcam user community

Verified customer

提交于 May 29 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Stomach)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization
No
Specification
Stomach
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 May 03 2018

Application
IHC - Wholemount
Sample
Mouse Tissue (intestine organoids)
Specification
intestine organoids
Username

Abcam user community

Verified customer

提交于 Mar 29 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH6
Permeabilization
No
Specification
colon
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Normal buffered formalin
Username

Abcam user community

Verified customer

提交于 Mar 17 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (small intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH6
Permeabilization
No
Specification
small intestine
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin
Username

Abcam user community

Verified customer

提交于 Mar 17 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (cancerous liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
cancerous liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 17 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (beta cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate PH 6
Permeabilization
No
Specification
beta cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 07 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1
Permeabilization
No
Specification
pancreas
Fixative
formalin 4%
Username

Dr. Emmanuel Bouchaert

Verified customer

提交于 Jan 06 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreas)
Antigen retrieval step
None
Permeabilization
No
Specification
Pancreas
Blocking step
Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF
Username

Premier Laboratory

Verified customer

提交于 May 04 2016

We recommend ab25693. This is currently the only chromogranin antibody for which we have canine IHC data. A collaborator tested it on sections of formalin-fixed paraffin-embedded canine small intestine and saw strong positive staining of a sub-populati...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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