Anti-Chromogranin A抗体(ab15160)


  • 产品名称Anti-Chromogranin A抗体
    参阅全部 Chromogranin A 一抗
  • 描述
    兔多克隆抗体to Chromogranin A
  • 经测试应用适用于: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human, Monkey
  • 免疫原

    Recombinant fragment from the C-terminal (Human)

  • 阳性对照
    • Pancreas



Our Abpromise guarantee covers the use of ab15160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 21383188
WB 1/100.
ICC/IF 1/100.
IHC-P 1/400.


  • 功能Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
  • 序列相似性Belongs to the chromogranin/secretogranin protein family.
  • 翻译后修饰Sulfated on tyrosine residues and/or contains sulfated glycans.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • 细胞定位Secreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt
  • 数据库链接
  • 形式According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
  • 别名
    • beta Granin antibody
    • CgA antibody
    • CHG A antibody
    • Chga antibody
    • Chromogranin A parathyroid secretory protein 1 antibody
    • Chromogranin A precursor antibody
    • ChromograninA antibody
    • CMGA_HUMAN antibody
    • ER-37 antibody
    • Pancreastatin antibody
    • Parastatin antibody
    • Parathyroid secretory protein 1 antibody
    • Pituitary secretory protein I antibody
    • SP I antibody
    • SP-I antibody
    • SP1 antibody
    • SPI antibody
    • Vasostatin antibody
    • Vasostatin I antibody
    • Vasostatin II antibody
    see all

Anti-Chromogranin A antibody 图像

  • ab15160 at 1/250 staining human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed before incubation with the antibody for 30 minutes at 25°C. An HRP conjugated rabbit polyclonal was used as the secondary.

    See Abreview

  • ab15160 staining Chromogranin A in mouse gastric gland cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton ×100 and blocking with 10% serum for 45min at 200C was performed. Samples were incubated with primary antibody (1/100) for 18 hours at 4°C. A Texas Red ®-conjugated goat polyclonal to rabbit IgG was used at dilution 1/200 as secondary antibody.

    See Abreview

  • ab15160 (1/100) staining Chromogranin A in paraffin-embbeded mouse pancreas tissue sections. Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in citrate buffer pH 6.0 and blocking (10 minutes, 1.5% serum). An HRP-conjugated goat polyclonal was used as the secondary. For further experimental details please refer to abreview.

    See Abreview

  • ab15160 staining Chromogranin A in mouse colon tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formalin fixation before heat mediated antigen retrieval with 10mM citric acid buffer and then the sample was blocked for 45 minutes at 20°C. The primary antibody (1/100) was incubated with sample in 10% serum for 18 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution 1/200 as secondary antibody.

    See Abreview

  • developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds
    Chromogranin A is easily fragmented and both bands observed in the Western Blot above are believed to correspond to Chromogranin A.

Anti-Chromogranin A antibody (ab15160)参考文献

This product has been referenced in:
  • Stancikova J  et al. NKD1 marks intestinal and liver tumors linked to aberrant Wnt signaling. Cell Signal 27:245-56 (2015). IHC ; Mouse . Read more (PubMed: 25446263) »
  • Mogoanta SS  et al. A nonfunctional neuroendocrine tumor of the pancreas - a case report. Rom J Morphol Embryol 56:511-9 (2015). IHC ; Human . Read more (PubMed: 26193221) »

See all 22 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Pancreas)
Antigen retrieval step None
Permeabilization No
Specification Pancreas
Blocking step Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative 10% NBF

Dr. Faye Doherty

Verified customer

提交于 May 04 2016

We recommend ab25693. This is currently the only chromogranin antibody for which we have canine IHC data. A collaborator tested it on sections of formalin-fixed paraffin-embedded canine small intestine and saw strong positive staining of a sub-populati...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Colon)
Specification Colon
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM Citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Feb 08 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry these 2 products did not perform as stated on the datasheet and for the inconvenience this h...

Read More

Thank you for your inquiry.

The immunogen sequence for ab15160 proprietary information, but I am happy to confirm it is located within aa 220-457 of P10645 (Uniprot). The epitope has not been mapped, thus the specific binding site is unknown....

Read More

Thank you for your enquiry.

I am sorry to confirm we do not have any experimental data regarding the cross-reactivity ofab15160 with serpinin, parastatin or catastatin. There is 100% homology between the immunogen and all three cleavage prod...

Read More

Thank you for contacting us.

The length of the immunogen used to raise Anti-Chromogranin A antibody (ab15160) is 194 aa.

Please let me know if there is anything else I can help you with.

Thank you for contacting us.

I am afraid the immunogen sequence for this product is confidential, it is located within aa 250-457 of P10645 (Uniprot). The epitope has not been mapped, thus the specific binding site is unknown.

I hop...

Read More

Merci de nous avoir contactés. Nous sommes désolés d'apprendre que le produit ab15160 lot 958010 que vous avez reçu ne fonctionne pas comme attendu. Je vais transmettre au laboratoire les détails de votre protocole. Ces informations no...

Read More
Application Western blot
Sample Rat Cell lysate - whole cell (Colonic epithelium)
Loading amount 15 µg
Specification Colonic epithelium
Gel Running Conditions Reduced Denaturing (gel 10%)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C

Miss. Laura Wrzosek

Verified customer

提交于 Mar 31 2011