Chromatin Extraction试剂盒(ab117152)


  • 产品名称Chromatin Extraction试剂盒
    参阅全部 Chromatin Extraction 试剂盒
  • 测试
    1 x 100 test
  • 样品类型
    Tissue, Adherent cells, Suspension cells
  • 检测时间
    1h 00m
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: all Mammals
  • 产品概述

    Chromatin Extraction Kit (ab117152) is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.


  • 经测试应用适用于: Functional Studiesmore details



    Our Abpromise guarantee covers the use of ab117152 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Chromatin Extraction Kit 图像

    • ChIP analysis of RNA polymerase II enriched in GAPDH and MLH1 promoters with chromatin extract prepared from formaldehyde fixed colon cancer cells (2x105) using ab117152.
    • Chromatin was extracted from 3x106 mouse neuroblastoma cells, fixed for 10min at RT in 0.1% formaldehyde. Cells were sonicated before last centrifugation, and following centrifugation, cells were treated with proteinase K & RNAse A.

      Chromatin yield was 4 µg chromatin/106 cells.

      Lane 1: 1kb DNA ladder Lane 2: 300 - 650bp DNA fragments
      Image courtesy of anonymous Abreview


    Chromatin Extraction Kit (ab117152)参考文献

    ab117152 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your inquiry.

    We have two Nuclear Extraction Kits in our catalog at the moment:

    ab113474 (or use the following:

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    Under these conditions it should be fine to reduce the amount of working extraction buffer so that the chromatin is less dilute however sonication using a water bath sonicator may be necessary to improve rupture of the nuclear membrane and release of c...

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    In general the chromatin fraction is 90% pure but it also depends on additional factors such as the cell/tissue type, and the chromatin extraction procedure itself such as how effectively the cytoplasmic fraction is removed.

    Likewise, t...

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    I can confirm that the chromatin fraction is 90% pure in general but it also depends on additional factors such as the cell/tissue type, and the chromatin extraction itself such as how effectively the cytoplasmic fraction is removed.

    The extraction buffer of this kit contains a low concentration of EDTA which should not significantly affect combination use of micrococcal nuclease with this kit as Ca++ concentration is 5 mM.

    The size can be checked by directly running the sheared chromatin as seen in the attached file. If DNA will be purified. The following quick method may be useful: a) Use 10 uL sample and add 40 uL H2O b) Reverse cross-link by adding 2 uL of 5 M ...

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    In general, the chromatin is easy to be sonicated to 200-1000 bps. If the chromatin extracts can not be broken down fully by sonication, it may be due to insufficient time length, power intensity, foaming of chromatin solution during sonication etc. th...

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    I hope the sonication step is helpful. I look forward to hearing how things go!

    In general, this kit could generate about 4 ug/1 million cells of chromatin or 2 ug of DNA (histone:DNA in chromatin is about 1.8-2 :1). Based on the described conditions by the customer, the relatively low yield of DNA seems mainly due to that nucleus...

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    Thank you for your enquiry. My colleague Karen asked me to follow up with you.

    How has it been determined that the chromatin is in the pellet rather than the supernatant?

    Thanks in advance for the additional information!