Anti-CHREBP抗体(HRP) (ab81958)

概述

  • 产品名称Anti-CHREBP抗体(HRP)
    参阅全部 CHREBP 一抗
  • 描述
    兔多克隆抗体to CHREBP (HRP)
  • 偶联物HRP
  • 特异性A customer has observed some cross-reactivity with MLX-interacting protein (Q9HAP2). Although the exact sequence is proprietary, the immunogen for ab81958 shows 75% homology with this protein.
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human CHREBP aa 800-852 (C terminal). The exact sequence is proprietary.
    Database link: NP_116569.1

  • 阳性对照
    • Human hepatocyte lysate, human hepatocytes, liver nuclear extracts from well fed rats, and colon epithelium.

性能

应用

Our Abpromise guarantee covers the use of ab81958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Predicted molecular weight: 95 kDa.
IHC-P Use at an assay dependent dilution.
ICC/IF 1/100 - 1/500.

靶标

  • 功能Transcriptional repressor. Binds to the canonical and non-canonical E box sequences 5'-CACGTG-3'.
  • 组织特异性Expressed in liver, heart, kidney, cerebellum and intestinal tissues.
  • 疾病相关Note=WBSCR14 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of WBSCR14 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • carbohydrate response element binding protein antibody
    • bHLHd14 antibody
    • Carbohydrate responsive element binding protein antibody
    • Class D basic helix-loop-helix protein 14 antibody
    • MIO antibody
    • MLX interacting protein like antibody
    • Mlx interactor antibody
    • MLX-interacting protein-like antibody
    • MLXIPL antibody
    • MONDOB antibody
    • WBS14_HUMAN antibody
    • WBSCR 14 antibody
    • WBSCR14 antibody
    • Williams Beuren syndrome chromosome region 14 antibody
    • Williams Beuren syndrome chromosome region 14 protein antibody
    • Williams-Beuren syndrome chromosomal region 14 protein antibody
    • WS basic helix loop helix leucine zipper protein antibody
    • WS basic-helix-loop-helix leucine zipper protein antibody
    • WS bHLH antibody
    • WS-bHLH antibody
    see all

Anti-CHREBP antibody (HRP) 图像

  • anti CHREBP (unconjugated form of ab81958), staining CHREBP in colon epithelium (40X) by immunohistochemistry.
  • anti CHREBP (unconjugated form of ab81958) + human hepatocyte lysate at 20 µg

    Predicted band size : 95 kDa
    Observed band size : 95 kDa
  • anti CHREBP (unconjugated form of ab81958), at a 1/100 dilution, staining CHREBP in human hepatocyte by Immunofluorescence. Overlay staining of ChREBP (rhodamine red) and DAPI nuclear staining (blue).
  • All lanes : anti CHREBP (unconjugated form of ab81958), at 1/1000 dilution

    Lane 1 : liver nuclear extracts from well fed rats at 10 µg
    Lane 2 : liver nuclear extracts from well fed rats at 5 µg
    Lane 3 : liver nuclear extracts from well fed rats at 1 µg
    Lane 4 : liver nuclear extracts from well fed rats at 0.5 µg


    Predicted band size : 95 kDa
    Observed band size : 95 kDa

Anti-CHREBP antibody (HRP) (ab81958)参考文献

ab81958 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (liver and fat tissue)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 50 µg
Specification liver and fat tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Feb 17 2016


Pour visualiser l’HRP il faut ajouter un substrat qui, lorsqu'il est oxydé par l'HRP, change de couleur ou produit de la lumière qui peut être détecté.
De nombreux substrats pour HRP peuvent être utilisés :
HRP catalyse la conversion des...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"