概述

  • 产品名称Anti-Chp1抗体- ChIP Grade
  • 描述
    兔多克隆抗体to Chp1 - ChIP Grade
  • 经测试应用适用于: ChIP, WBmore details
  • 种属反应性
    与反应: Schizosaccharomyces pombe
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 900 to the C-terminus of Schizosaccharomyces pombe Chp1.

    (Peptide available as ab19117.)

  • 阳性对照
    • This antibody gave a positive signal in S. pombe whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab18191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use 2.5µg for 107 cells.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 109 kDa (predicted molecular weight: 109 kDa).

靶标

  • 相关性Chp1 a chromodomain protein associates with the S. pombe mating type locus and telomeres. Chp1 localization to heterochromatin depends on its chromodomain and the C-terminal domain of the protein. Chp1 and centromeric siRNAs are components of the RITS (RNA-induced initiation of transcriptional gene silencing) complex. The association of RITS with centromeres is linked to Dicer activity, which is required to cleave centromeric transcripts to produce short interfering RNAs that actively recruit components of heterochromatin to centromeres.
  • 细胞定位Nuclear
  • 数据库链接
    • 别名
      • Chromo domain containing protein 1 antibody
      • Chromodomain protein Chp1 antibody

    Anti-Chp1 antibody - ChIP Grade 图像

    • All lanes : Anti-Chp1 antibody - ChIP Grade (ab18191) at 1 µg/ml

      Lane 1 : S. pombe chp1Δ lysate
      Lane 2 : S. pombe wildtype lysate


      Predicted band size : 109 kDa
      Observed band size : 109 kDa
      Additional bands at : 102 kDa (possible degradation product).

      This image is courtesy of Danesh Moazed's Laboratory, Harvard, Boston.

      ab18191 specifically recognises Chp1 at 109 kDa in wildtype S. pombe lysate but not in chp1Δ lysate.
    • X-ChIP performed with ab18191 on Schizosaccharomyces pombe Cell lysate (whole cell). 5µl antibody were used per 500µl of chromatin/cell lysate according to standard S. pombe ChIP protocols. Protein-A Sepharose was used to recover the ChIPs. In the positive control the researcher specifically induced silencing of the ura4+ gene. For the negative control  the researcher performed multiplex PCR with primers amplifying ura4+ and an un-silenced gene. In addition, act1+ was amplified in a separate PCR reaction.  Chp1 only binds to ura4+ (and only if silencing is induced).

      This image is courtesy of an Abreview submitted by Marc B? on 9 February 2006

      See Abreview

    • Anti-Chp1 antibody - ChIP Grade (ab18191) at 1 µg/ml + S.pombe Whole Cell Lysate at 20 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 109 kDa
      Observed band size : 115 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 75&42 kDa. We are unsure as to the identity of these extra bands.

    Anti-Chp1 antibody - ChIP Grade (ab18191)参考文献

    This product has been referenced in:
    • Creamer KM  et al. The Mi-2 homolog Mit1 actively positions nucleosomes within heterochromatin to suppress transcription. Mol Cell Biol 34:2046-61 (2014). Read more (PubMed: 24662054) »
    • Hiriart E  et al. Mmi1 RNA surveillance machinery directs RNAi complex RITS to specific meiotic genes in fission yeast. EMBO J 31:2296-308 (2012). ChIP . Read more (PubMed: 22522705) »

    See all 10 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application ChIP
    Sample Schizosaccharomyces pombe Cell lysate - whole cell
    Type Cross-linking (X-ChIP)
    Duration of cross-linking step: 0 second(s)
    Specification of the cross-linking agent: 3% Formaldehyde
    Detection step Semiquantitative PCR
    Positive control I specifically induce silencing of the ura4+ gene. I see enrichment for this gene in ChIPs performed with antibodies against H3K9me2, Ago1, Swi6 and, as I show here, Chp1. All these proteins are recruited to transcriptional silent loci as for example centromeric DNA repeats. The Chp1 antibody precipitates chromatin very well (see figure )-it gives me the strongest signal among all the antibodies I used so far.
    Negative control I perform multiplex PCR with primers amplifying ura4+ and an un-silenced gene. In addition, act1+ was amplified in a separate PCR reaction. As you can see, Chp1 only binds to ura4+ (and only if silencing is induced).
    Username

    Dr. Marc B?

    Verified customer

    提交于 Feb 09 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"