Anti-Choline Oxidase抗体(Biotin) (ab34528)

概述

  • 产品名称
    Anti-Choline Oxidase抗体(Biotin)
    参阅全部 Choline Oxidase 一抗
  • 描述
    山羊多克隆抗体to Choline Oxidase (Biotin)
  • 偶联物
    Biotin
  • 经测试应用
    适用于: WB, ELISA, Immunomicroscopy, Dot blotmore details
  • 种属反应性
    与反应: Goat
  • 免疫原

    Choline Oxidase from Alcaligenes species.

  • 常规说明

    10-20 Biotin molecules per goat IgG molecule.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride

    BSA is IgG and Protease free
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    This product is purified by a multi-step process including delipidation, salt fractionation, ion exchange chromatography and extensive dialysis against buffer.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab34528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
Immunomicroscopy
Dot blot
  • 应用说明
    Dot: Use at an assay dependent dilution.
    ELISA: 1/4000 - 1/20000.
    This product has been assayed against 1.0µg of Choline Oxidase in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic-acid]) as a substrate for 30 minutes at RT.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.
    Suitable for use in other antibody based assays using streptavidin or avidin conjugates.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      Responsible for phosphatidylcholine synthesis via the CDP-choline pathway. Also exhibits ethanolamine kinase activity but at 14% efficiency compared with choline. ATP + choline = ADP + phosphocholine. Belongs to the choline/ethanolamine kinase family.
    • 细胞定位
      Cytoplasm.
    • 别名
      • L3130 antibody
      • Choline kinase antibody
      • CKI antibody
      • CKI1 antibody
      • L9606.8 antibody
      • YLR133W antibody
      see all

    实验方案

    文献

    ab34528 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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