Anti-Choline kinase alpha抗体- Aminoterminal end (ab38290)

概述

  • 产品名称
    Anti-Choline kinase alpha抗体- Aminoterminal end
    参阅全部 Choline kinase alpha 一抗
  • 描述
    兔多克隆抗体to Choline kinase alpha - Aminoterminal end
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected within aa 50~150 of human Choline kinase alpha.

  • 阳性对照
    • Mouse heart tissue lysate.
  • 常规说明


    This antibody recognises the N-terminal region of CHK.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • Primary antibody说明
    This antibody recognises the N-terminal region of CHK.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab38290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Detects a band of approximately 48 kDa (predicted molecular weight: 52 kDa).
ELISA 1/1000.

靶标

  • 相关性
    The dominant pathway for biosynthesis of phosphatidylcholine occurs through the CDP-choline pathway. Choline kinase alpha (CHK) is the first enzyme in the pathway and may thereby play an upstream regulatory role in lipid transport and metabolism. CHK additionally catalyzes phosphorylation of ethanolamine.
  • 细胞定位
    Cytoplasmic
  • 数据库链接
  • 别名
    • CHETK alpha antibody
    • CHETK-alpha antibody
    • CHK antibody
    • CHKA antibody
    • CK antibody
    • CKI antibody
    • EK antibody
    • Ethanolamine kinase antibody
    see all

Anti-Choline kinase alpha antibody - Aminoterminal end 图像

实验方案

Anti-Choline kinase alpha antibody - Aminoterminal end (ab38290)参考文献

This product has been referenced in:
  • Arlauckas SP  et al. Near infrared fluorescent imaging of choline kinase alpha expression and inhibition in breast tumors. Oncotarget 8:16518-16530 (2017). Read more (PubMed: 28157707) »
  • Huang C  et al. Activation of choline kinase by extracellular Ca2+ is Ca(2+)-sensing receptor, Galpha12 and Rho-dependent in breast cancer cells. Cell Signal 21:1894-900 (2009). WB ; Human . Read more (PubMed: 19716891) »

See all 3 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer cell line)
Loading amount
30 µg
Specification
breast cancer cell line
Gel Running Conditions
Reduced Denaturing (8-16%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dorit Grunberger

Verified customer

提交于 Aug 05 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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