Anti-Choline kinase alpha抗体(ab88053)

概述

  • 产品名称
    Anti-Choline kinase alpha抗体
    参阅全部 Choline kinase alpha 一抗
  • 描述
    兔多克隆抗体to Choline kinase alpha
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human Choline kinase alpha.

    (Peptide available as ab98290.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Human Breast Tissue; Mouse Lung Tissue; Mouse Brain Tissue; Rat Brain Tissue; MCF7 Whole Cell; HepG2 Whole Cell; HeLa Whole Cell; Jurkat Whole Cell; A549, Human Glioblastoma Whole Cell.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab88053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

Anti-Choline kinase alpha antibody 图像

  • All lanes : Anti-Choline kinase alpha antibody (ab88053) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : Human breast tissue lysate - total protein (ab30090)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 6 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ICC/IF image of ab88053 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88053, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • All lanes : Anti-Choline kinase alpha antibody (ab88053) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse lung normal tissue lysate - total protein (ab29297)
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 72 kDa,78 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • Immunocytochemistry/ Immunofluorescence analysis of human glioblastoma cells labeling Choline kinase alpha with ab88053 at 1/250 dilution. Cells were fixed in paraformaldehyde and permeabilized with TritonX-100. A goat anti-rabbit Alexa Fluor594 was used as the secondary antibody at 1/1000 dilution.

    See Abreview

  • All lanes : Anti-Choline kinase alpha antibody (ab88053) at 1/1000 dilution

    Lane 1 : Choline kinase alpha knockdown Human Glioblastoma whole cell lysate
    Lane 2 : Human Glioblastoma (Control) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    goat-anti-rabbit IRDye800CW at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 52 kDa


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-Choline kinase alpha antibody (ab88053)参考文献

This product has been referenced in:
  • Wong MT & Chen SS Hepatitis C Virus Subverts Human Choline Kinase-a To Bridge Phosphatidylinositol-4-Kinase IIIa (PI4KIIIa) and NS5A and Upregulates PI4KIIIa Activation, Thereby Promoting the Translocation of the Ternary Complex to the Endoplasmic Reticulum for Viral Replication. J Virol 91:N/A (2017). Read more (PubMed: 28566381) »
  • Zhang L  et al. CHKA mediates the poor prognosis of lung adenocarcinoma and acts as a prognostic indicator. Oncol Lett 12:1849-1853 (2016). WB ; Human . Read more (PubMed: 27588131) »

See all 5 Publications for this product

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Lung tumor and normal lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: HIER Citrate Pressure Cooker
Permeabilization
No
Specification
Lung tumor and normal lung
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Formaldehyde
Username

Sofya Osharovich

Verified customer

提交于 Mar 15 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Glioblastoma)
Permeabilization
Yes - TritonX-100
Specification
Glioblastoma
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 21 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma)
Gel Running Conditions
Reduced Denaturing (4-12 % gradient gel)
Loading amount
50 µg
Specification
Glioblastoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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