Anti-Choline kinase alpha抗体(ab88053)
Key features and details
- Rabbit polyclonal to Choline kinase alpha
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Choline kinase alpha抗体
参阅全部 Choline kinase alpha 一抗 -
描述
兔多克隆抗体to Choline kinase alpha -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Zebrafish -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Recombinant Human Choline kinase alpha protein (ab114521) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: Human Breast Tissue; Mouse Lung Tissue; Mouse Brain Tissue; Rat Brain Tissue; MCF7 Whole Cell; HepG2 Whole Cell; HeLa Whole Cell; Jurkat Whole Cell; A549, Human Glioblastoma Whole Cell.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88053于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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相关性
The dominant pathway for biosynthesis of phosphatidylcholine occurs through the CDP-choline pathway. Choline kinase alpha (CHK) is the first enzyme in the pathway and may thereby play an upstream regulatory role in lipid transport and metabolism. CHK additionally catalyzes phosphorylation of ethanolamine. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 1119 Human
- Entrez Gene: 12660 Mouse
- Entrez Gene: 29194 Rat
- Entrez Gene: 497433 Zebrafish
- Omim: 118491 Human
- SwissProt: P35790 Human
- SwissProt: O54804 Mouse
- SwissProt: Q01134 Rat
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别名
- CHETK alpha antibody
- CHETK-alpha antibody
- CHK antibody
see all
图片
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All lanes : Anti-Choline kinase alpha antibody (ab88053) at 1 µg/ml
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : Human breast tissue lysate - total protein (ab30090)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 6 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes -
ICC/IF image of ab88053 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88053, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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All lanes : Anti-Choline kinase alpha antibody (ab88053) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 72 kDa, 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
Immunocytochemistry/ Immunofluorescence analysis of human glioblastoma cells labeling Choline kinase alpha with ab88053 at 1/250 dilution. Cells were fixed in paraformaldehyde and permeabilized with TritonX-100. A goat anti-rabbit Alexa Fluor594 was used as the secondary antibody at 1/1000 dilution.
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All lanes : Anti-Choline kinase alpha antibody (ab88053) at 1/1000 dilution
Lane 1 : Choline kinase alpha knockdown Human Glioblastoma whole cell lysate
Lane 2 : Human Glioblastoma (Control) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : goat-anti-rabbit IRDye800CW at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 52 kDa
Exposure time: 1 minute
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab88053 被引用在 13 文献中.
- Tavasoli M et al. A mouse model of inherited choline kinase β-deficiency presents with specific cardiac abnormalities and a predisposition to arrhythmia. J Biol Chem 298:101716 (2022). PubMed: 35151687
- Tavasoli M et al. Mechanism of action and therapeutic route for a muscular dystrophy caused by a genetic defect in lipid metabolism. Nat Commun 13:1559 (2022). PubMed: 35322809
- Patel SH et al. The Impact of Nicotine along with Oral Contraceptive Exposure on Brain Fatty Acid Metabolism in Female Rats. Int J Mol Sci 23:N/A (2022). PubMed: 36555717
- Raikundalia S et al. MicroRNA-367-3p induces apoptosis and suppresses migration of MCF-7 cells by downregulating the expression of human choline kinase a. Oncol Lett 21:183 (2021). PubMed: 33574922
- Hua W et al. Metabolic Reprogramming of Mammary Epithelial Cells during TGF-β-Induced Epithelial-to-Mesenchymal Transition. Metabolites 11:N/A (2021). PubMed: 34564442