Anti-Choline Acetyltransferase抗体- C-terminal (ab85609)

概述

  • 产品名称Anti-Choline Acetyltransferase抗体- C-terminal
    参阅全部 Choline Acetyltransferase 一抗
  • 描述
    兔多克隆抗体to Choline Acetyltransferase - C-terminal
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Rat Choline Acetyltransferase.

    (Peptide available as ab97420.)

  • 阳性对照
    • This antibody gave a positive signal in both Rat Cortex and Mouse Cholinergic nucleus Tissue Lysates.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab85609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).

靶标

  • 功能Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
  • 疾病相关Myasthenic syndrome, congenital, 6, presynaptic
  • 序列相似性Belongs to the carnitine/choline acetyltransferase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acetyl CoA choline O acetyltransferase antibody
    • Acetyl CoA:choline O acetyltransferase antibody
    • ChAT antibody
    • CHOACTase antibody
    • Choline acetylase antibody
    • choline acetyltransferase antibody
    • Choline O acetyltransferase antibody
    • Choline O-acetyltransferase antibody
    • CLAT_HUMAN antibody
    • CMS1A antibody
    • CMS1A2 antibody
    • EC 2.3.1.6 antibody
    • OTTHUMP00000019583 antibody
    • OTTHUMP00000019584 antibody
    see all

Anti-Choline Acetyltransferase antibody - C-terminal 图像

  • Anti-Choline Acetyltransferase antibody - C-terminal (ab85609) at 1 µg/ml + Cortex (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 72 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 18 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Anti-Choline Acetyltransferase antibody - C-terminal (ab85609) at 1 µg/ml + Cholinergic nucleus Mouse Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 72 kDa
    Observed band size : 76 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 78 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

Anti-Choline Acetyltransferase antibody - C-terminal (ab85609)参考文献

This product has been referenced in:
  • Moghimi E  et al. Dietary Vitamin D3 Restriction Exacerbates Disease Pathophysiology in the Spinal Cord of the G93A Mouse Model of Amyotrophic Lateral Sclerosis. PLoS One 10:e0126355 (2015). WB ; Mouse . Read more (PubMed: 26020962) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Brain
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Nov 04 2013

Thank you very much for your interest in our products. The following antibodies are tested and guaranteed for IHC-P in rat. Unfortunately, we do not have blocking peptide for these antibodies in our catalogue. ab68779 Click here (or use...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"