Anti-Choline Acetyltransferase抗体(ab6168)

概述

  • 产品名称Anti-Choline Acetyltransferase抗体
    参阅全部 Choline Acetyltransferase 一抗
  • 描述
    兔多克隆抗体to Choline Acetyltransferase
  • 特异性Extensive immunohistochemistry indicates localisation to cholinergic nervous system only. Cross reactivity: This antibody is known to react with rat and guinea pig. Cross reactivity with other species has not been tested yet.
  • 经测试应用适用于: ICC/IF, ELISA, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Guinea pig
    预测可用于: Chicken, Human, Non Human Primates不与反应: Sheep
  • 免疫原

    Synthetic peptide:

    H-GLFSSYRLPGHTQDTLVAQKSS

    conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 168-189 of Choline Acetyltransferase.

  • 常规说明This antibody appears to have identical characteristics to those described by Benecke et al (1993). The antigen is the same. It is an excellent reagent for immunohistochemistry for both peripheral and central nervous system, staining both cell bodies and nerve terminals.

性能

应用

Our Abpromise guarantee covers the use of ab6168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. See Abreview.
ELISA 1/1000.
IHC-P Use at an assay dependent concentration. PubMed: 19422789
IHC-Fr 1/2000. Fix using formaldehyde. Perfusion fix the tissue with Zamboni fixative and post-fix (1-2hrs according to Masliukov PM & Timmermans) in 4% formaldehyde (4% formaldehyde with or without 15% picric acid is a very good fixative, it should also work with Bouin fixative too).Cryoprotect the tissue by direct immersion in 30% sucrose (at +4C). Use on free floating sections for best results.
This antibody does not work with glutaraldehyde fixation.

靶标

Anti-Choline Acetyltransferase antibody 图像

  • ab6168 staining rat stomach tissue sections by immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde. The primary antibody was diluted 1/200 and incubated with sample for 24 hours. A Texas Red® conjugated goat polyclonal (IgG whole molecule) to rabbit IgG, diluted 1/400 was used  as secondary. 

    See Abreview

  • ab6168 staining cells in rat brain section by ICC/IF. Cells were PFA fixed and blocked in 10% serum for 1 hour at 25°C. The primary antibody was diluted 1/200 in 10% donkey serum and incubated with the sample for 24 hours at 25°C. A FITC-conjugated donkey polyclonal to rabbit IgG, diluted 1/200 was used the secondary.

    See Abreview

Anti-Choline Acetyltransferase antibody (ab6168)参考文献

This product has been referenced in:
  • Tu JL  et al. APOE 4 polymorphism results in early cognitive deficits in an EAE model. Biochem Biophys Res Commun 384:466-70 (2009). WB, IHC-P ; Mouse . Read more (PubMed: 19422789) »
  • Masliukov PM & Timmermans JP Immunocytochemical properties of stellate ganglion neurons during early postnatal development. Histochem Cell Biol 122:201-9 (2004). Read more (PubMed: 15338227) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human Mueller cells (MIO-M1))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH 6
Permeabilization No
Specification human Mueller cells (MIO-M1)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative 10% NBF
Username

Abcam user community

Verified customer

提交于 Feb 17 2016

Application Western blot
Sample Rhesus monkey Tissue lysate - whole (macaque retina)
Gel Running Conditions Reduced Denaturing (4-15%)
Loading amount 100 µg
Specification macaque retina
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Monica Sauter

Verified customer

提交于 Jan 11 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cynomolgus Monkey Tissue sections (macaque retina)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH 6
Permeabilization No
Specification macaque retina
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 29 2015

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 21°C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.25 % Triton-x ind 0.1M PB
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 10 2013

Thank you for your reply.

We have not validated ab6168 for use on perfused-fixed frozen sections (IHC-FoFr), and it would not be guaranteed for use in that application. The alternative antibody ab18736 is known to work well on perfused-fixed ...

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Thank you for your email. I am sorry that this antibody is giving a weak signal. Could you please clarify a few points of your protocol?

1) What type of samples were tested? Were these rat brain sections or some other type of tissue? How long...

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Thank you for contacting Abcam. I am sorry that you are having problems with ab6168 in IHC-P. I found the following reference that used the antibody successfully in IHC-P on mouse tissue: Tu JL et al. APOE 4 polymorphism results in early cognitive defi...

Read More

Thank you very much for your interest in our products. The following antibodies are tested and guaranteed for IHC-P in rat. Unfortunately, we do not have blocking peptide for these antibodies in our catalogue. ab68779 Click here (or use...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Stomach)
Specification Stomach
Fixative Paraformaldehyde
Permeabilization No
Username

Prof. Marcin Arciszewski

Verified customer

提交于 Oct 27 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Li-Hsien Lin

Verified customer

提交于 Oct 10 2008

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"