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Our Abpromise guarantee covers the use of ab6168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. See Abreview.|
|IHC-P||Use at an assay dependent concentration. PubMed: 19422789|
|IHC-Fr||1/2000. Fix using formaldehyde. Perfusion fix the tissue with Zamboni fixative and post-fix (1-2hrs according to Masliukov PM & Timmermans) in 4% formaldehyde (4% formaldehyde with or without 15% picric acid is a very good fixative, it should also work with Bouin fixative too).Cryoprotect the tissue by direct immersion in 30% sucrose (at +4C). Use on free floating sections for best results.
This antibody does not work with glutaraldehyde fixation.
Immunohistochemistry analysis of free-floating rat forebrain tissue labelling Choline Acetyltransferase with ab6168 at a dilution of 1/100.
ab6168 staining rat stomach tissue sections by immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde. The primary antibody was diluted 1/200 and incubated with sample for 24 hours. A Texas Red® conjugated goat polyclonal (IgG whole molecule) to rabbit IgG, diluted 1/400 was used as secondary.
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