Anti-Choline Acetyltransferase抗体(ab18736)

概述

  • 产品名称Anti-Choline Acetyltransferase抗体
    参阅全部 Choline Acetyltransferase 一抗
  • 描述
    羊多克隆抗体to Choline Acetyltransferase
  • 经测试应用适用于: IHC-FoFr, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Guinea pig, Human, Pig
    预测可用于: Chicken
  • 免疫原

    Synthetic peptide:

    GLFSSYRLPGHTQDTLVAQKSS

    conjugated to KLH, corresponding to amino acids 167-188 of Pig Choline Acetyltransferase.

性能

应用

Our Abpromise guarantee covers the use of ab18736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/1000.

Use on perfusion fixed tissue (1% sodium Nitrite in 0.1M TBS, pH, 7.4 for 20 min then 4% formaldehyde containing 15% picric acid in 0.1M TBS,pH, 7.4 for 20min). Please note sections must be post fixed if the tissue is cut freshly.

IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 83 kDa.
ICC/IF 1/100. PubMed: 18393628

靶标

Anti-Choline Acetyltransferase antibody 图像

  • ab18736 staining Choline Acetyltransferase in mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton X-100 and blocked with 3% donkey serum in 0.3% Triton X-100/TBS for 1 hour at 21°C. The sample was incubated with primary antibody (1/1000 in 1% donkey serum + 0.3% Triton X-100/TBS) at 4°C for 24 hours. An Alexa Fluor® 594-conjugated donkey anti-sheep polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical detection of ChAT in rat basal forebrain using Sheep anti ChAT (ab18736).

    Rat was perfused with 1% sodium Nitrite in 0.1M TBS, pH, 7.4 for 20 min. and then fixed with 4% formaldehyde containing 15% picric acid in 0.1M TBS, pH, 7.4 for 30 min. The brain was then cryoprotected in 30% overnight and cut into 40 nm free-floating sections. Sections were washed 2x with TBS for 20 min. and passed through asending ethanol and finally transferred to 0.1 M TBS for rehydration for 1h.

Anti-Choline Acetyltransferase antibody (ab18736)参考文献

This product has been referenced in:
  • Yu B  et al. Mechanisms Underlying Footshock and Psychological Stress-Induced Abrupt Awakening From Posttraumatic "Nightmares". Int J Neuropsychopharmacol N/A:N/A (2015). Rat . Read more (PubMed: 26591007) »
  • Hadzipasic M  et al. Selective degeneration of a physiological subtype of spinal motor neuron in mice with SOD1-linked ALS. Proc Natl Acad Sci U S A 111:16883-8 (2014). IHC ; Mouse . Read more (PubMed: 25385594) »

See all 7 Publications for this product

Product Wall

Application IHC - Wholemount
Sample Rat Tissue (Myenteric plexus from rat colon)
Specification Myenteric plexus from rat colon
Username

Abcam user community

Verified customer

提交于 Aug 23 2016

Application IHC - Wholemount
Sample Mouse Tissue (myenteric plexus from colon)
Specification myenteric plexus from colon
Username

Abcam user community

Verified customer

提交于 Aug 22 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Donkey Serum in 0.3% Triton X-100/TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Antigen retrieval step None
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
Username

Ms. Carla Cabral

Verified customer

提交于 Mar 28 2014

Thank you very much for your interest in our products. The following antibodies are tested and guaranteed for IHC-P in rat. Unfortunately, we do not have blocking peptide for these antibodies in our catalogue. ab68779 Click here (or use...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (brain CA1)
Specification brain CA1
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization Yes - 0.1% IGEPAL CA630
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 Apr 10 2009

Thank you for your enquiry. Please find below the answers to your questions: Q1. was the fix of 4% formaldehyde containing 15% picric acid in 0.1M TBS,pH, 7.4 for 30 min done after the brain was extracted, and if so was the whole brain simply immer...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"