Cholesterol/Cholesteryl Ester Assay试剂盒- Detection (ab102515)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell Lysate, Tissue Lysate
概述
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产品名称
Cholesterol/Cholesteryl Ester Assay试剂盒- Detection
参阅全部 Cholesterol/Cholesteryl Ester 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell Lysate, Tissue Lysate -
检测类型
Quantitative -
检测时间
1h 00m -
产品概述
Cholesterol/Cholesteryl Ester Assay Kit ab102515 uses a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both using a colorimetric method.
This assay kit uses an alternative method compared to our most popular Cholesterol/ Cholesteryl Ester Assay Kit ab65359 (which relies on a cholesterol oxidase based method).
In this assay kit, free cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at 450 nm.
The assay can detect free or total cholesterol depending upon whether cholesterol esterase is used to hydrolyze cholesterol esters present.
Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters).
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K623 Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II. K623-100 is the same size as the 100 test size of ab102515.
Cholesterol is an essential molecule in all animal life. It has been involved in both normal development and diseases.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Assay Buffer II 1 x 25ml Cholesterol Enzyme Mix 1 vial Cholesterol Esterase 1 vial Cholesterol Standard 1 x 100µl Developer Solution III 1 vial -
研究领域
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相关性
Cholesterol is an essential molecule in all animal life. It is involved in both normal development and diseases. A high level of serum cholesterol is an indicator for diseases such as heart disease. Cholesteryl ester is an ester of cholesterol. The ester bond is formed between the carboxylate group of a fatty acid and the hydroxyl group of cholesterol.
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图片
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab102515 被引用在 19 文献中.
- Du Q et al. FASN promotes lymph node metastasis in cervical cancer via cholesterol reprogramming and lymphangiogenesis. Cell Death Dis 13:488 (2022). PubMed: 35597782
- Jagirdar G et al. Re-Expression of Tafazzin Isoforms in TAZ-Deficient C6 Glioma Cells Restores Cardiolipin Composition but Not Proliferation Rate and Alterations in Gene Expression. Front Genet 13:931017 (2022). PubMed: 35957687
- Kusuyama J et al. Placental superoxide dismutase 3 mediates benefits of maternal exercise on offspring health. Cell Metab 33:939-956.e8 (2021). PubMed: 33770509
- Templehof H et al. Zebrafish mutants provide insights into Apolipoprotein B functions during embryonic development and pathological conditions. JCI Insight 6:N/A (2021). PubMed: 34236046
- Wei X et al. Knockdown of PEX16 Induces Autophagic Degradation of Peroxisomes. Int J Mol Sci 22:N/A (2021). PubMed: 34360754