Cholesterol/ Cholesteryl Ester Quantitation试剂盒(ab65359)

概述

  • 产品名称Cholesterol/ Cholesteryl Ester Quantitation试剂盒
    参阅全部 Cholesterol/Cholesteryl Ester 试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • 检测类型Quantitative
  • 检测时间
    1h 00m
  • 产品概述

    Cholesterol/ Cholesteryl Ester Quantitation Kit (ab65359) provides a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both by colorimetric or fluorometric methods. Majority of the cholesterol in blood is in the form of cholesteryl esters, which can be hydrolyzed to cholesterol by cholesterol esterase. Cholesterol is then oxidized by cholesterol oxidase to yield H2O2 which reacts with a sensitive cholesterol probe to produce color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The assay detects total cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase or free cholesterol in the absence of cholesterol esterase in the reaction. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total (cholesterol plus cholesteryl esters).
    Visit our FAQs page for tips and troubleshooting.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Cholesterol is an essential molecule in all animal life. It is involved in both normal development and diseases. A high level of serum cholesterol is an indicator for diseases such as heart disease. Cholesteryl ester is an ester of cholesterol. The ester bond is formed between the carboxylate group of a fatty acid and the hydroxyl group of cholesterol.

应用

Our Abpromise guarantee covers the use of ab65359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Cholesterol/ Cholesteryl Ester Quantitation Kit 图像

  • Cholesterol and cholesteryl ester were quantified colourimetrically in biological fluids (dilution range 1:10-1:100; 50 minutes  incubation) and measured in duplicates (+/- SD).

  • Detection of Cholesterol/Cholesteryl Ester Using Cholesterol Quantitation Kit (ab65359). Cholesterol/Cholesteryl Ester was quantified using the kit by colorimetric (A) and fluorometric (B) methods according to the kit instructions. Background from the control reaction (without cholesterol) has been subtracted from each value. Note: Fluorometric assay is over 10 fold more sensitive than colorimetric assay.

实验方案

Cholesterol/ Cholesteryl Ester Quantitation Kit (ab65359)参考文献

This product has been referenced in:
  • DiStefano MT  et al. The Lipid Droplet Protein Hypoxia-inducible Gene 2 Promotes Hepatic Triglyceride Deposition by Inhibiting Lipolysis. J Biol Chem 290:15175-84 (2015). Read more (PubMed: 25922078) »
  • Gao F  et al. Radiation-driven lipid accumulation and dendritic cell dysfunction in cancer. Sci Rep 5:9613 (2015). Read more (PubMed: 25923834) »

See all 11 Publications for this product

Product Wall

ab65359 will extract total lipids, a large portion of which is from the plasma membrane in a normal cell.



Ich habe im Labor nachgefragt und habe die folgende Antwort erhalten:

"Ideally, cells/tissues should be frozen before organic lipid extraction. Alternately, they can be stored after evaporation but this can lead to lipids sticki...

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We do not suggest extracting triglyceride and cholesterol in an unique extraction step with two layers. We have not tested this with each kit and cannot guarantee the effectiveness. Triglyceride is simply solubilized in detergent-water and total l...

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This kit is sold based on number of tests. A ‘test’ simply refers to a single assay well. The number of wells that contain sample, control or standard will be each counted as a test. Therefore this kit will be enough to perform less th...

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Triton can generate background and hence we recommend NP-40. If it is absolutely essential for you to use Triton, you can run a blank using the triton solution to see what the interference is like.

We do carry NP-40 as ab142227 (link below) ...

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Hydrogen peroxide release is proportional to the cholesterol esterification reaction. H2O2 reacts with the probe to generate color at a downstream step.

Thank you for contacting us.

Yes, you would see a pellet and an organic phase which contains all the cholesterol. This organic phase may seem to have multiple layers, so you did right in collecting all. This should be a max of 200 µl. S...

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Trypsin is a protease so it shouldn't have any effect on the cholesterol in the sample.

Thank you for contacting us.

We have not tested the kit this way, and are not sure whether it would work for ergosterol/ergosterol ester. Please do not hesitate to contact us if you need any more advice or information.

Thanks for your inquiry. The datasheet in step 2, calls for dissolving dried lipids in 200 µl of Cholesterol Assay Buffer by sonicating or vortexing until homogeneous. At this stage, it is OK if the solution becomes cloudy. After extraction, one ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"