Anti-Cholera Toxin抗体[B041M] (ab62970)

概述

  • 产品名称Anti-Cholera Toxin抗体[B041M]
    参阅全部 Cholera Toxin 一抗
  • 描述
    小鼠单克隆抗体[B041M] to Cholera Toxin
  • 特异性ab62970 reacts with cholera toxin beta subunit.
  • 经测试应用适用于: ELISAmore details
  • 免疫原

    Purified beta subunit of Cholera Toxin.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明>90% pure (SDS-PAGE).
  • 克隆单克隆
  • 克隆编号B041M
  • 骨髓瘤Sp2/0
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab62970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.

靶标

  • 相关性The holotoxin (choleragen) consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself. After binding to gangliosides GM1 in lipid rafts, through the subunit B pentamer, the holotoxin and the gangliosides are internalized. The holotoxin remains bound to GM1 until arrival in the ER. The A subunit has previously been cleaved in the intestinal lumen but the A1 and A2 chains have remained associated. In the ER, the A subunit disulfide bridge is reduced, the A1 chain is unfolded by the PDI and disassembled from the rest of the toxin. Then, the membrane-associated ER oxidase ERO1 oxidizes PDI, which releases the unfolded A1 chain. The next step is the retrotranslocation of A1 into the cytosol. This might be mediated by the protein-conducting pore SEC61. Upon arrival in the cytosol, A1 refolds and avoids proteasome degradation. In one way or another, A1 finally reaches its target and induces toxicity.
  • 细胞定位Secreted
  • 别名
    • ctxA antibody
    • Cholera enterotoxin B chain antibody
    • Cholera enterotoxin gamma chain antibody
    • Cholera enterotoxin subunit A antibody
    • Cholera enterotoxin subunit B antibody
    • Choleragenoid antibody
    • ctxA antibody
    • ctxB antibody
    • toxA antibody
    • toxB antibody
    see all

Anti-Cholera Toxin antibody [B041M] (ab62970)参考文献

ab62970 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"