Anti-Cholera Toxin抗体(ab51572)

概述

  • 产品名称
    Anti-Cholera Toxin抗体
    参阅全部 Cholera Toxin 一抗
  • 描述
    兔多克隆抗体to Cholera Toxin
  • 特异性
    ab51572 is specific for the b subunit of Cholera Toxin.
  • 经测试应用
    适用于: ELISAmore details
  • 免疫原

    Purified Choleragenoid (beta subunit).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab51572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
  • 应用说明
    ELISA: Titre 1/2000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      The holotoxin (choleragen) consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself. After binding to gangliosides GM1 in lipid rafts, through the subunit B pentamer, the holotoxin and the gangliosides are internalized. The holotoxin remains bound to GM1 until arrival in the ER. The A subunit has previously been cleaved in the intestinal lumen but the A1 and A2 chains have remained associated. In the ER, the A subunit disulfide bridge is reduced, the A1 chain is unfolded by the PDI and disassembled from the rest of the toxin. Then, the membrane-associated ER oxidase ERO1 oxidizes PDI, which releases the unfolded A1 chain. The next step is the retrotranslocation of A1 into the cytosol. This might be mediated by the protein-conducting pore SEC61. Upon arrival in the cytosol, A1 refolds and avoids proteasome degradation. In one way or another, A1 finally reaches its target and induces toxicity.
    • 细胞定位
      Secreted
    • 别名
      • ctxA antibody
      • Cholera enterotoxin B chain antibody
      • Cholera enterotoxin gamma chain antibody
      • Cholera enterotoxin subunit A antibody
      • Cholera enterotoxin subunit B antibody
      • Choleragenoid antibody
      • ctxA antibody
      • ctxB antibody
      • toxA antibody
      • toxB antibody
      see all

    Anti-Cholera Toxin antibody (ab51572)参考文献

    ab51572 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
    Sample
    Rat Tissue sections (Dorsal Root Ganglion)
    Specification
    Dorsal Root Ganglion
    Permeabilization
    Yes - PBS-Tx
    Fixative
    Paraformaldehyde
    Username

    Mr. Matthew Thakur

    Verified customer

    提交于 Feb 28 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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