概述

  • 产品名称Anti-CHMP4B抗体
    参阅全部 CHMP4B 一抗
  • 描述
    兔多克隆抗体to CHMP4B
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 143-192 (EEISTAISKP VGFGEEFDED ELMAELEELE QEELDKNLLE ISGPETVPLP) of Human CHMP4B (NP_789782).

性能

应用

Our Abpromise guarantee covers the use of ab105767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration. PubMed: 21926173

靶标

  • 功能Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding. Via its interaction with PDCD6IP involved in HIV-1 p6-and p9-dependent virus release.
  • 组织特异性Widely expressed. Expressed at higher level in heart and skeletal muscle. Also expressed in brain, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes.
  • 疾病相关Defects in CHMP4B are the cause of cataract posterior polar type 3 (CTPP3) [MIM:605387]. A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity.
  • 序列相似性Belongs to the SNF7 family.
  • 结构域The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
  • 细胞定位Cytoplasm > cytosol. Late endosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Charged multivesicular body protein 4b antibody
    • CHM4B_HUMAN antibody
    • chmp4b antibody
    • Chromatin-modifying protein 4b antibody
    • hSnf7-2 antibody
    • hVps32-2 antibody
    • SNF7 homolog associated with Alix 1 antibody
    • SNF7-2 antibody
    • Vacuolar protein sorting-associated protein 32-2 antibody
    • Vps32-2 antibody
    see all

Anti-CHMP4B antibody 图像

  • ab105767 staining CHMP4B (red) in HeLa cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed with 4% paraformaldehyden and after three washes in PBS, cells were permeabilized with PBS containing 0.5% Triton X-100 in blocking solution (PBS containing 5% goat pre-immune serum) for 30 minutes. ab105767 was diluted in blocking solution and then incubated for 1 hour. After washing in PBS, cells were incubated for 1 hour with the secondary antibody.
  • Anti-CHMP4B antibody (ab105767) at 1 µg/ml + lysate prepared from THP-1 cells

    Predicted band size : 25 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

Anti-CHMP4B antibody (ab105767)参考文献

This product has been referenced in:
  • Bodon G  et al. Charged Multivesicular Body Protein 2B (CHMP2B) of the Endosomal Sorting Complex Required for Transport-III (ESCRT-III) Polymerizes into Helical Structures Deforming the Plasma Membrane. J Biol Chem 286:40276-86 (2011). WB, ICC/IF ; Human . Read more (PubMed: 21926173) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Human Cell lysate - whole cell (liver carcinoma cell line)
Specification liver carcinoma cell line
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Nov 05 2014

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