Anti-CHMP4A抗体(ab67058)
Key features and details
- Mouse polyclonal to CHMP4A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CHMP4A抗体 -
描述
小鼠多克隆抗体to CHMP4A -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human CHMP4A aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- transfected 293T cells lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab67058于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 25 kDa. Use BSA for blocking (see Abreview)
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 25 kDa. Use BSA for blocking (see Abreview) |
靶标
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相关性
Component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In the ESCRT-III complex, it probably serves as an acceptor for ESCRT-I complex on endosomal membranes. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargos of viral proteins, via the association of CHMP4 proteins with PDCD6IP/AIP1, a protein directly recruited by HIV-1 p6 protein that functions at sites of viral Gag assembly and budding. -
细胞定位
Cytoplasmic: Cytoplasmic vesicle membrane. Membrane-associated - localizes to large vesicle-like structures. -
数据库链接
- Entrez Gene: 29082 Human
- GenBank: BC010893 Human
- Omim: BC010893 Human
- SwissProt: Q9BY43 Human
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别名
- C14orf123 antibody
- Charged multivesicular body protein 4a antibody
- Chromatin modifying protein 4a antibody
see all
图片
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All lanes : Anti-CHMP4A antibody (ab67058) at 1/500 dilution
Lane 1 : CHMP4A transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 25 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (2)
ab67058 被引用在 2 文献中.
- Jia J et al. Galectin-3 Coordinates a Cellular System for Lysosomal Repair and Removal. Dev Cell 52:69-87.e8 (2020). PubMed: 31813797
- Arii J et al. ESCRT-III mediates budding across the inner nuclear membrane and regulates its integrity. Nat Commun 9:3379 (2018). PubMed: 30139939