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Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human CHMP2B.
(Peptide available as ab27782.)
Our Abpromise guarantee covers the use of ab33174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. See Abreview.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa.|
|IHC-P||Use at an assay dependent concentration. PubMed: 20420883|
|ICC/IF||Use a concentration of 5 µg/ml.|
ab33174 staining CHMP2B in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary. The antibody produced a diffuse and widespread staining in many brain areas. The image shows the staining obtained in the mouse hippocampus.
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