概述

  • 产品名称Anti-CHMP2A抗体
    参阅全部 CHMP2A 一抗
  • 描述
    小鼠多克隆抗体to CHMP2A
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-222 of Human CHMP2A

  • 阳性对照
    • Transfected 293T cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.4
    Constituent: PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab67064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/500 - 1/1000. Predicted molecular weight: 25 kDa.
    Detection pair

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release.
    • 序列相似性Belongs to the SNF7 family.
    • 结构域The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
    • 细胞定位Late endosome membrane. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BC2 antibody
      • Charged multivesicular body protein 2a antibody
      • CHM2A_HUMAN antibody
      • CHMP2 antibody
      • CHMP2a antibody
      • Chromatin modifying protein 2a antibody
      • Chromatin-modifying protein 2a antibody
      • hVps2 1 antibody
      • hVps2-1 antibody
      • putative breast adenocarcinoma marker antibody
      • Putative breast adenocarcinoma marker BC-2 antibody
      • Putative breast adenocarcinoma marker BC2 antibody
      • Vacuolar protein sorting associated protein 2 1 antibody
      • Vacuolar protein sorting-associated protein 2-1 antibody
      • Vps2 1 antibody
      • VPS2 antibody
      • Vps2-1 antibody
      • VPS2A antibody
      see all

    Anti-CHMP2A antibody 图像

    • All lanes : Anti-CHMP2A antibody (ab67064) at 1/500 dilution

      Lane 1 : CHMP2A transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 25 kDa
      Observed band size : ~28 kDa (why is the actual band size different from the predicted?)

    Anti-CHMP2A antibody (ab67064)参考文献

    ab67064 has not yet been referenced specifically in any publications.

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