Anti-Chloride Channel 5抗体(ab97482)

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概述

  • 产品名称
    Anti-Chloride Channel 5抗体
    参阅全部 Chloride Channel 5 一抗
  • 描述
    兔多克隆抗体to Chloride Channel 5
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Guinea pig, Pig
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 62-363 of Human chloride channel 5 (NP_000075)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • A431 whole cell lysate, HepG2 and Raji lysates.

性能

应用

Our Abpromise guarantee covers the use of ab97482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 83 kDa.

靶标

  • 功能
    Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function.
  • 组织特异性
    Kidney. Moderately expressed in aortic vascular smooth muscle and endothelial cells, and at a slightly higher level in the coronary vascular smooth muscle.
  • 疾病相关
    Hypophosphatemic rickets, X-linked recessive
    Nephrolithiasis 2
    Nephrolithiasis 1
    Low molecular weight proteinuria with hypercalciuria and nephrocalcinosis
  • 序列相似性
    Belongs to the chloride channel (TC 2.A.49) family. ClC-5/CLCN5 subfamily.
    Contains 2 CBS domains.
  • 翻译后修饰
    Ubiquitinated by NEDD4L in the presence of albumin; which promotes endocytosis and proteasomal degradation.
  • 细胞定位
    Golgi apparatus membrane. Endosome membrane. Cell membrane.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • Chloride channel protein 5 antibody
    • Chloride channel voltage sensitive 5 antibody
    • Chloride transporter ClC-5 antibody
    • ClC-5 antibody
    • CLC5 antibody
    • CLCK2 antibody
    • CLCN5 antibody
    • CLCN5_HUMAN antibody
    • DENTS antibody
    • H(+)/Cl(-) exchange transporter 5 antibody
    • hCIC-K2 antibody
    • NPHL1 antibody
    • NPHL2 antibody
    • Voltage gated chloride ion channel CLCN5 antibody
    • XLRH antibody
    • XRN antibody
    see all

图片

  • Western blot - Anti-Chloride Channel 5 antibody (ab97482)
    Western blot - Anti-Chloride Channel 5 antibody (ab97482)
    Anti-Chloride Channel 5 antibody (ab97482) at 1/500 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 83 kDa



    7.5% SDS PAGE

实验方案

文献

ab97482 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab97482？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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2 reviews or Q&A

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Chloride Channel 5 antibody

 Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (LLCPK1)
Permeabilization
Yes - 0.3% Triton in PBS
Specification
LLCPK1
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde
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Dr. Sufyan Ali Sayyed

Verified customer

提交于 Oct 05 2016

Western blot abreview for Anti-Chloride Channel 5 antibody

 Inconclusive
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Cell lysate - whole cell (LLCPK1)
Gel Running Conditions
Reduced Denaturing (7.5)
Loading amount
50 µg
Specification
LLCPK1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Username

Dr. Sufyan Ali Sayyed

Verified customer

提交于 Oct 05 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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