Chloride Assay试剂盒(ab83372)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.4 mM
概述
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产品名称
Chloride Assay试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
检测类型
Quantitative -
灵敏度
> 0.4 mM -
范围
20 nmol/well - 120 nmol/well -
检测时间
0h 20m -
产品概述
Abcam's Chloride Assay Kit provides a quick, simple method for quantification of Chloride in a variety of biological samples. Blood and urine can be used directly after dilution with water. The assay is based upon the competition of Hg2+ and Fe2+ for TPTZ. The preferred Hg-TPTZ adduct exhibits no color. In the presence of Chloride, Hg2+ forms HgCl2 freeing up TPTZ which then binds the available Fe2+ giving a very intense absorbance with a λmax~ 620nm. The assay is linear in the range 20 to 120 nmol Chloride/well with detection sensitivity ~0.4 mM chloride.
Visit our FAQs page for tips and troubleshooting. -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K530 Chloride Colorimetric Assay Kit. K530-100 is the same size as the 100 test size of ab83372.
Chloride is the anionic form of chlorine. It is the most common of the anions found in living organisms. Chloride ions play a variety of important physiological roles. Chloride channels are found in a variety of cells and are responsible for setting resting cell membrane potential and regulating cell volume. In the nervous system, the action of glycine and GABA are related to chloride levels in specific neurons. Chloride is also instrumental in maintaining the acid-base balance in blood. The kidneys are instrumental in closely regulating serum chloride levels. There are a number of pathologies associated with defective chloride transport; the most well-known being Cystic Fibrosis, caused by a mutation in CFTR a membrane chloride transporter.
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平台
Microplate reader
性能
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存放说明
Store at room temperature. Please refer to protocols. -
组件 100 tests Chloride Reagent 1 x 15ml Chloride Standard 1 vial -
研究领域
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相关性
Chloride is the anionic form of chlorine. It is the most common of the anions found in living organisms. Chloride ions play a variety of important physiological roles. Chloride channels are found in a variety of cells and are responsible for setting resting cell membrane potential and regulating cell volume. In the nervous system, the action of glycine and GABA are related to chloride levels in specific neurons. Chloride is also instrumental in maintaining the acid-base balance in blood. The kidneys are instrumental in closely regulating serum chloride levels. There are a number of pathologies associated with defective chloride transport; the most well-known being Cystic Fibrosis, caused by a mutation in CFTR a membrane chloride transporter.
图片
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab83372 被引用在 3 文献中.
- Yang HS et al. Respiratory Safety Evaluation in Mice and Inhibition of Adenoviral Amplification in Human Bronchial Endothelial Cells Using a Novel Type of Chlorine Dioxide Gas Reactor. Toxics 10:N/A (2022). PubMed: 35051080
- Shu T et al. Depletion of Tissue-Specific Ion Transporters Causes Differential Expression of PRL Targets in Response to Increased Levels of Endogenous PRL. Front Endocrinol (Lausanne) 9:683 (2018). PubMed: 30515132
- Shu Y et al. The basal function of teleost prolactin as a key regulator on ion uptake identified with zebrafish knockout models. Sci Rep 6:18597 (2016). PubMed: 26726070