Anti-Chloramphenicol抗体(ab35658)

概述

  • 产品名称Anti-Chloramphenicol抗体
    参阅全部 Chloramphenicol 一抗
  • 描述
    羊多克隆抗体to Chloramphenicol
  • 特异性ab35658 is specific to Chloramphenicol (CAP, Cm).
  • 经测试应用适用于: ELISA, FPIAmore details
  • 免疫原

    Aromatic amino derivative of chloramphenicol (prepared by reduction of the nitro group by high-pressure hydrogenation) conjugated to KLH using the diazotisation procedure.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Prepared by Ammonium Sulphate fractionation.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab35658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
FPIA
  • 应用说明ELISA: 1/4000 - 1/500000.
    ab35658 was used in a simple indirect ELISA with 400 ng of Chloramphenicol-BSA per well. The titres detailed above were elucidated using an HRP labelled secondary antibody/TMB detection system.

    FPIA: 1/1500.
    When tested by polarisation fluoroimmunoassay, the final titre dilution was found to be 0.5 ml tracer + 0.5 ml diluted antibody. Tracer is aromatic amino derivative of chloramphenicol labelled by reaction with fluorescein isothiocyanate (10 nmol/L).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Chloramphenicol is an antibiotic that was derived from the bacterium Streptomyces venezuelae. It was the first antibiotic to be manufactured synthetically on a large scale. Chloramphenicol is effective against a wide variety of microorganisms, but due to serious side effects (eg damage to the bone marrow) in humans, it is usually reserved for the treatment of serious and life threatening infections (eg typhoid fever). It is also used in eye drops or ointment to treat bacterial conjunctivitis.

    Anti-Chloramphenicol antibody (ab35658)参考文献

    ab35658 has not yet been referenced specifically in any publications.

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