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corresponding to Human Chk2.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our Abpromise guarantee covers the use of ab32148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/20 - 1/50.|
|WB||1/1000 - 1/5000. Detects a band of approximately 62 kDa (predicted molecular weight: 61 kDa).|
Blocking/dilution buffer: 5% NFDM/TBST
ab32148 at 1/20 dilution immunoprecipitating Chk2 (phospho T68) in HEK-293 (human embryonic kidney) whole cell lysate observed at 70kDa (lanes 1 and 2).
Lane 1 (input): HEK-293 cells treated with UV at 60J/m2 for 2 hours. Whole cell lysate 10μg
Lane 2 (+): ab32148 + HEK-293 treated with UV at 60J/m2 for 2 hours. Whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32148 in HEK- treated with UV at 60J/m2 for 2 hours. Whole cell lysate
For western blotting, ab32148 at 1/500 dilution was used followed by ab131366 VeriBlot for IP (HRP) at 1/1000 as the secondary antibody.
Blocking and Diluting buffer and concentration: 5% NFDM/TBST.
Non-specific band : 35, 30 kDa
Blocking/Diluting buffer : 5% Milk
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"