概述

  • 产品名称Anti-Chk2抗体- C-terminal
    参阅全部 Chk2 一抗
  • 描述
    兔多克隆抗体to Chk2 - C-terminal
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Chk2 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • HL60 cell lysate and human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab70860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100. Detects a band of approximately 75 kDa (predicted molecular weight: 61 kDa).

靶标

  • 功能Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
  • 组织特异性High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
  • 疾病相关Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
    Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
    Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
    Contains 1 FHA domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated by PLK4.
  • 细胞定位Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDS 1 antibody
    • Cds1 antibody
    • Cds1 homolog antibody
    • Checkpoint kinase 2 antibody
    • Checkpoint like protein CHK2 antibody
    • CHEK 2 antibody
    • Chek2 antibody
    • Chk 2 antibody
    • CHK2 checkpoint homolog (S. pombe) antibody
    • CHK2 checkpoint homolog antibody
    • CHK2_HUMAN antibody
    • hCds1 antibody
    • HuCds 1 antibody
    • LFS 2 antibody
    • LFS2 antibody
    • PP1425 antibody
    • RAD 53 antibody
    • RAD53 antibody
    • Rad53 homolog antibody
    • Serine/threonine protein kinase Chk2 antibody
    • Serine/threonine-protein kinase Chk2 antibody
    see all

Anti-Chk2 antibody - C-terminal 图像

  • Anti-Chk2 antibody - C-terminal (ab70860) at 1/100 dilution + HL60 cell lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit

    Predicted band size : 61 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,35 kDa. We are unsure as to the identity of these extra bands.
  • ab70860 at 1/50 dilution, staining Chk2 in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.

Anti-Chk2 antibody - C-terminal (ab70860)参考文献

This product has been referenced in:
  • Mahyous Saeyd SA  et al. Hyperactive Cdc2 kinase interferes with the response to broken replication forks by trapping S.pombe Crb2 in its mitotic T215 phosphorylated state. Nucleic Acids Res 42:7734-47 (2014). Read more (PubMed: 24861625) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab70860.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"