Anti-Chk1 (phospho S345)抗体(ab47318)

概述

  • 产品名称
    Anti-Chk1 (phospho S345)抗体
    参阅全部 Chk1 一抗
  • 描述
    兔多克隆抗体to Chk1 (phospho S345)
  • 经测试应用
    适用于: ICC/IF, IHC-Fr, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Drosophila melanogaster
  • 免疫原

    The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 345 (S-F-SP-Q-P).

  • 阳性对照
    • Human breast carcinoma tissue
  • 常规说明


    Chk1 (phospho-Ser345) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 345.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    The antibody was purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Primary antibody说明
    Chk1 (phospho-Ser345) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 345.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab47318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200. See Abreview.
IHC-Fr Use at an assay dependent concentration. PubMed: 20978077Fixed in -20°C methanol.
IHC-P 1/50 - 1/100.
ELISA 1/10000.

靶标

Anti-Chk1 (phospho S345) antibody 图像

  • ab47318 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide.
  • ab47318 (1/200) detecting Chk1 in HeLa cells in conjunction with a goat anti-rabbit secondary antibody conjugated to Cy3® (red). Cells were also counterstained with DAPI in order to highlight the nucleus (blue), treated with Bleomycin and incubated with an antibody against Histone H2AX in order to create and expose DNA double strand breaks (green). Please refer to abreviews for further details.

    See Abreview

Anti-Chk1 (phospho S345) antibody (ab47318)参考文献

This product has been referenced in:
  • Wang CY  et al. DNA-PK/Chk2 induces centrosome amplification during prolonged replication stress. Oncogene N/A:N/A (2014). Read more (PubMed: 24662822) »
  • Zhou J  et al. A kinome screen identifies checkpoint kinase 1 (CHK1) as a sensitizer for RRM1-dependent gemcitabine efficacy. PLoS One 8:e58091 (2013). Human . Read more (PubMed: 23483975) »

See all 8 Publications for this product

Product Wall

Thanks for your email.
Please feel free to call me between hours of 8:30am and 5pm EST. Our Scientific Support is available from 8am - 8pm EST and you can feel free to speak to any of my colleagues about these issues as well.
I look forward t...

Read More

Thank you for completing our recent survey. As you have noted technical difficulties with two antibodies from Abcam, I just wanted to follow up to see how I might help to rectify any issues.
To help me assist you most efficiently, it would be help...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MRC5VA Lung Epithelial Fibroblasts)
Loading amount
15 µg
Specification
MRC5VA Lung Epithelial Fibroblasts
Gel Running Conditions
Reduced Denaturing (9%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mr. Darren Arbon

Verified customer

提交于 Apr 14 2011

Thank you for your enquiry. All the information that we have about the protocol used by K. McManus is already stated on the review. At Abcam, we always give our customers all the information that we have at hand and I am afraid that there is no othe...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Apr 19 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册