Anti-Chk1 (phospho S301)抗体(ab61023)

概述

  • 产品名称
    Anti-Chk1 (phospho S301)抗体
    参阅全部 Chk1 一抗
  • 描述
    兔多克隆抗体to Chk1 (phospho S301)
  • 特异性
    ab61023 detects endogenous levels of Chk1 only when phosphorylated at serine 301.
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of serine 301 (D-F-SP-P-V).

  • 阳性对照
    • Extracts from 293 cells

性能

应用

Our Abpromise guarantee covers the use of ab61023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/40000.
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).

靶标

图片

  • All lanes : Anti-Chk1 (phospho S301) antibody (ab61023) at 1/500 dilution

    Lane 1 : Extracts from 293 cells
    Lane 2 : Extracts from 293 cells with Immunizing peptide


    Predicted band size : 54 kDa
    Observed band size : 50,~100 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab61023 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human mammary cell)
Loading amount
50000 cells
Specification
Human mammary cell
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Colon cell line)
Loading amount
20 µg
Specification
Colon cell line
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Dec 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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