Anti-Chk1 (phospho S301)抗体(ab61023)

概述

  • 产品名称Anti-Chk1 (phospho S301)抗体
    参阅全部 Chk1 一抗
  • 描述
    兔多克隆抗体to Chk1 (phospho S301)
  • 特异性ab61023 detects endogenous levels of Chk1 only when phosphorylated at serine 301.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of serine 301 (D-F-SP-P-V).

  • 阳性对照
    • Extracts from 293 cells

性能

应用

Our Abpromise guarantee covers the use of ab61023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/40000.
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).

靶标

Anti-Chk1 (phospho S301) antibody 图像

  • All lanes : Anti-Chk1 (phospho S301) antibody (ab61023) at 1/500 dilution

    Lane 1 : Extracts from 293 cells
    Lane 2 : Extracts from 293 cells with Immunizing peptide


    Predicted band size : 54 kDa
    Observed band size : 50,~100 kDa (why is the actual band size different from the predicted?)

Anti-Chk1 (phospho S301) antibody (ab61023)参考文献

ab61023 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human mammary cell)
Loading amount 50000 cells
Specification Human mammary cell
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

提交于 Apr 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Colon cell line)
Loading amount 20 µg
Specification Colon cell line
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

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Verified customer

提交于 Dec 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"