Anti-Chk1 (phospho S296)抗体[EPR915] (ab79758)


  • 产品名称Anti-Chk1 (phospho S296)抗体[EPR915]
    参阅全部 Chk1 一抗
  • 描述
    兔单克隆抗体[EPR915] to Chk1 (phospho S296)
  • 特异性The antibody only detects Chk1 phosphorylated at Serine 296.
  • 经测试应用适用于: WBmore details
    不适用于: ICC/IF or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding Serine 296 of human Chk1

  • 阳性对照
    • 283T cell lysate treated with UV and AP; HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab79758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

For unpurified use at 1/1000 - 1/2000

  • 应用说明Is unsuitable for ICC/IF or IHC-P.
  • 靶标

    • Information by UniProt
    • 数据库链接
    • 别名
      • C85740 antibody
      • Cell cycle checkpoint kinase antibody
      • Checkpoint , S. pombe, homolog of, 1 antibody
      • Checkpoint kinase 1 antibody
      • Checkpoint kinase 1 homolog (S. pombe) antibody
      • CHEK 1 antibody
      • Chek1 antibody
      • Chk 1 antibody
      • Chk1 antibody
      • CHK1 checkpoint homolog (S. pombe) antibody
      • CHK1_HUMAN antibody
      • EC antibody
      • rad27 antibody
      • Serine/threonine protein kinase Chk1 antibody
      • Serine/threonine-protein kinase CHK1 antibody
      • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
      see all

    Anti-Chk1 (phospho S296) antibody [EPR915] 图像

    • All lanes : Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758) at 1/1500 dilution

      Lane 1 : Untreated 293 cell lysate
      Lane 2 : Treated with Calyculin A 293 cell lysate

      Lysates/proteins at 10 µg per lane.

      Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

      Predicted band size : 54 kDa
    • All lanes : Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758) at 1/1000 dilution (Unpurified)

      Lane 1 : Untreated 293 cell Lysate
      Lane 2 : Treated with Calyculin 293 cell Lysate

      Lysates/proteins at 10 µg per lane.

      Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

      Predicted band size : 54 kDa

    Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758)参考文献

    This product has been referenced in:
    • Bryant C  et al. Inhibition of the checkpoint kinase Chk1 induces DNA damage and cell death in human Leukemia and Lymphoma cells. Mol Cancer 13:147 (2014). WB . Read more (PubMed: 24913641) »
    • Rawlinson R & Massey AJ ?H2AX and Chk1 phosphorylation as predictive pharmacodynamic biomarkers of Chk1 inhibitor-chemotherapy combination treatments. BMC Cancer 14:483 (2014). WB ; Human . Read more (PubMed: 24996846) »

    See all 6 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (colon cell line)
    Loading amount 20 µg
    Specification colon cell line
    Gel Running Conditions Reduced Denaturing (4-12%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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    提交于 Dec 29 2010