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RabMAb

Anti-Chk1 (phospho S296)抗体[EPR915] (ab79758)

概述

  • 产品名称
    Anti-Chk1 (phospho S296)抗体[EPR915]
    参阅全部 Chk1 一抗
  • 描述
    兔单克隆抗体[EPR915] to Chk1 (phospho S296)
  • 特异性
    The antibody only detects Chk1 phosphorylated at Serine 296.
  • 经测试应用
    适用于: WBmore details
    不适用于: ICC/IF or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    corresponding to Human Chk1.

  • 阳性对照
    • 283T cell lysate treated with UV and AP; HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab79758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

For unpurified use at 1/1000 - 1/2000

  • 应用说明
    Is unsuitable for ICC/IF or IHC-P.
  • 靶标

    • Information by UniProt
    • 数据库链接
    • 别名
      • C85740 antibody
      • Cell cycle checkpoint kinase antibody
      • Checkpoint , S. pombe, homolog of, 1 antibody
      • Checkpoint kinase 1 antibody
      • Checkpoint kinase 1 homolog (S. pombe) antibody
      • CHEK 1 antibody
      • Chek1 antibody
      • Chk 1 antibody
      • Chk1 antibody
      • CHK1 checkpoint homolog (S. pombe) antibody
      • CHK1_HUMAN antibody
      • EC 2.7.11.1 antibody
      • rad27 antibody
      • Serine/threonine protein kinase Chk1 antibody
      • Serine/threonine-protein kinase CHK1 antibody
      • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
      see all

    图片

    • All lanes : Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758) at 1/1500 dilution

      Lane 1 : Untreated 293 cell lysate
      Lane 2 : Treated with Calyculin A 293 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

      Predicted band size : 54 kDa
    • All lanes : Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758) at 1/1000 dilution (Unpurified)

      Lane 1 : Untreated 293 cell Lysate
      Lane 2 : Treated with Calyculin 293 cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

      Predicted band size : 54 kDa

    实验方案

    文献

    This product has been referenced in:
    • Karanika S  et al. Targeting DNA Damage Response in Prostate Cancer by Inhibiting Androgen Receptor-CDC6-ATR-Chk1 Signaling. Cell Rep 18:1970-1981 (2017). Read more (PubMed: 28228262) »
    • Massey AJ Tumour growth environment modulates Chk1 signalling pathways and Chk1 inhibitor sensitivity. Sci Rep 6:35874 (2016). Read more (PubMed: 27775084) »

    See all 8 Publications for this product

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (colon cell line)
    Loading amount
    20 µg
    Specification
    colon cell line
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 29 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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