重组Anti-Chk1 (phospho S296)抗体[EPR915] (ab79758)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR915] to Chk1 (phospho S296)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Chk1 (phospho S296)抗体[EPR915]
参阅全部 Chk1 一抗 -
描述
兔单克隆抗体[EPR915] to Chk1 (phospho S296) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- 283T cell lysate treated with UV and AP; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.1% BSA, 9.85% Tris glycine -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR915 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79758于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
For unpurified use at 1/1000 - 1/2000 |
说明 |
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WB
1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). For unpurified use at 1/1000 - 1/2000 |
靶标
- Information by UniProt
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数据库链接
- Entrez Gene: 1111 Human
- Omim: 603078 Human
- SwissProt: O14757 Human
- Unigene: 24529 Human
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别名
- C85740 antibody
- Cell cycle checkpoint kinase antibody
- Checkpoint , S. pombe, homolog of, 1 antibody
see all
图片
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All lanes : Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758) at 1/1500 dilution
Lane 1 : Untreated 293 cell lysate
Lane 2 : Treated with Calyculin A 293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution
Predicted band size: 54 kDa -
All lanes : Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758) at 1/1000 dilution (Unpurified)
Lane 1 : Untreated 293 cell Lysate
Lane 2 : 293 cell lysate, treated with Calyculin
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution
Predicted band size: 54 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab79758 被引用在 20 文献中.
- Wang YC et al. Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination. Cell Rep 42:112296 (2023). PubMed: 36961817
- Luo Q et al. Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity. Cell Biol Toxicol N/A:N/A (2022). PubMed: 35142956
- Wang L et al. Long Noncoding RNA Solute Carrier Family 25 Member 21 Antisense RNA 1 Inhibits Cell Malignant Behaviors and Enhances Radiosensitivity of Gastric Cancer Cells by Upregulating Synuclein Gamma Expression. Tohoku J Exp Med 257:225-239 (2022). PubMed: 35444105
- Redwood AB et al. The cytosolic iron-sulfur cluster assembly (CIA) pathway is required for replication stress tolerance of cancer cells to Chk1 and ATR inhibitors. NPJ Breast Cancer 7:152 (2021). PubMed: 34857765
- Laroche-Clary A et al. ATR Inhibition Broadly Sensitizes Soft-Tissue Sarcoma Cells to Chemotherapy Independent of Alternative Lengthening Telomere (ALT) Status. Sci Rep 10:7488 (2020). PubMed: 32366852