Anti-Chk1 (phospho S286)抗体(ab62243)

概述

  • 产品名称
    Anti-Chk1 (phospho S286)抗体
    参阅全部 Chk1 一抗
  • 描述
    兔多克隆抗体to Chk1 (phospho S286)
  • 宿主
    Rabbit
  • 特异性
    ab62243 detects endogenous levels of Chk1 only when phosphorylated at serine 286.
  • 经测试应用
    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of serine 286 (S-E-SP-P-S).

  • 阳性对照
    • HeLa cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab62243 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab62243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

靶标

  • Information by UniProt
  • 数据库链接
  • 别名
    • C85740 antibody
    • Cell cycle checkpoint kinase antibody
    • Checkpoint , S. pombe, homolog of, 1 antibody
    • Checkpoint kinase 1 antibody
    • Checkpoint kinase 1 homolog (S. pombe) antibody
    • CHEK 1 antibody
    • Chek1 antibody
    • Chk 1 antibody
    • Chk1 antibody
    • CHK1 checkpoint homolog (S. pombe) antibody
    • CHK1_HUMAN antibody
    • EC 2.7.11.1 antibody
    • rad27 antibody
    • Serine/threonine protein kinase Chk1 antibody
    • Serine/threonine-protein kinase CHK1 antibody
    • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
    see all

图片

  • ab62243 at 1/500 - 1/1000 dilution staining Chk1 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

文献

ab62243 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human mammary cell)
Loading amount
50000 cells
Specification
Human mammary cell
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (colon cell line)
Loading amount
25 µg
Specification
colon cell line
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 28 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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