Anti-Chk1 (phospho S280)抗体(ab47560)

概述

  • 产品名称
    Anti-Chk1 (phospho S280)抗体
    参阅全部 Chk1 一抗
  • 描述
    兔多克隆抗体to Chk1 (phospho S280)
  • 特异性
    This antibody detects endogenous levels of Chk1 only when phosphorylated at serine 280.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of serine 280 (V-T-SP-G-G).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab47560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 54 kDa.
ELISA 1/20000.

靶标

图片

  • All lanes : Anti-Chk1 (phospho S280) antibody (ab47560) at 1/500 dilution

    Lane 1 : HT29 cells, untreated
    Lane 2 : HT29 cells treated with UV


    Predicted band size : 54 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab47560 has not yet been referenced specifically in any publications.

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