鸡Anti-Rabbit IgG H&L (TRITC) (ab6826)

概述

  • 产品名称鸡Anti-Rabbit IgG H&L (TRITC)
    参阅全部 IgG 二抗
  • 靶标种属Rabbit
  • 经测试应用适用于: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • 免疫原

    Rabbit IgG, whole molecule

  • 偶联物TRITC. Ex: 547nm, Em: 572nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
  • 共轭说明Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG
  • 克隆多克隆
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Immunomicroscopy
Flow Cyt
ICC/IF
IHC-P
IHC-Fr
ELISA
  • 应用说明suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
  • Chicken Anti-Rabbit IgG H&L (TRITC) (ab6826)参考文献

    ab6826 has not yet been referenced specifically in any publications.

    Product Wall

    I'm sorry to hear you are experiencing problems with ab6826, the fact that a "no primary" control gave you high background does indeed point towards a non-specificity of a damaged vial. Could you please clarify what dilution of this secondary antibody ...

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    All the information avaliable for this product is stated on the datasheet. The datasheet reads "This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose". Therefore this antibody will be...

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    There should be no difference between any of these secondaries; they will all work similarly well. However, if you wish to reduce background with the human cells then we suggest using secondaries that have been pre-adsorbed against human serum, thus, ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"