概述

  • 产品名称Anti-CHI3L1抗体
    参阅全部 CHI3L1 一抗
  • 描述
    兔多克隆抗体to CHI3L1
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human CHI3L1.

    (Peptide available as ab88218.)

  • 阳性对照
    • This antibody gave a positive signal in THP1 Whole Cell Lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab77528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment.
  • 组织特异性Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.
  • 疾病相关A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7) [MIM:611960]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis.
  • 序列相似性Belongs to the glycosyl hydrolase 18 family.
  • 翻译后修饰Glycosylated.
  • 细胞定位Secreted > extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 39 kDa synovial protein antibody
    • ASRT7 antibody
    • Cartilage glycoprotein 39 antibody
    • CGP-39 antibody
    • CGP39 antibody
    • CH3L1_HUMAN antibody
    • CHI3L1 antibody
    • chitinase 3 like 1 (cartilage glycoprotein 39) antibody
    • chitinase 3 like 1 antibody
    • Chitinase 3 like protein 1 precursor antibody
    • chitinase antibody
    • Chitinase-3-like protein 1 antibody
    • Chondrocyte protein YKL40 antibody
    • GP 39 antibody
    • GP-39 antibody
    • GP39 antibody
    • HC gp39 antibody
    • HCGP 3P antibody
    • hCGP-39 antibody
    • HCgp39 antibody
    • YKL 40 antibody
    • YKL-40 antibody
    • YKL40 antibody
    • YYL 40 antibody
    see all

Anti-CHI3L1 antibody 图像

  • Anti-CHI3L1 antibody (ab77528) at 1 µg/ml + THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • IHC image of CHI3L1 staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77528, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab77528 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab77528 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CHI3L1 antibody (ab77528)参考文献

ab77528 has not yet been referenced specifically in any publications.

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