Chemiluminescent HRP Substrate (膜) (ab5801)

Submit an Abreview Q&A (4)Specific References (2)
Customer reviews and FAQs

概述

  • 产品名称Chemiluminescent HRP Substrate (膜)
  • 产品概述

    A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.

     

    The Abcam Chemiluminescent HRP Substrate (membrane) is very sensitive and great care must be taken to optimize the individual assay components (antibodies, conjugates, solid phase, etc.) to minimize background reactivity associated with nonspecific immunochemical reactivities

  • 说明

    Prepare 1x substrate by mixing 1 ml of the chemiluminescent reagent with 2 ml of the chemiluminescent reaction buffer. Mix well and protect from light. More or less of the 1x substrate can be prepared using the same ratio.

    Stability: Stable for a minimum of 18 months when stored at 2-8ºC in the original container and protected from light. The 1x diluted reagent is stable for several hours at room temperature when protected from light.

  • 经测试应用适用于: WBmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab5801 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use at an assay dependent concentration.

    Incubate membrane in chemiluminescent substrate for 1 minute then rinse off.

    Chemiluminescent HRP Substrate (membrane) (ab5801)参考文献

    This product has been referenced in:
    • Wang P  et al. Decorin reduces hypertrophic scarring through inhibition of the TGF-ß1/Smad signaling pathway in a rat osteomyelitis model. Exp Ther Med 12:2102-2108 (2016). WB . Read more (PubMed: 27698699) »
    • Pacini S  et al. Constitutive expression of pluripotency-associated genes in mesodermal progenitor cells (MPCs). PLoS One 5:e9861 (2010). Read more (PubMed: 20360837) »

    See all 2 Publications for this product

    发表研究结果有使用 ab5801？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    4 Abreviews or Q&A

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    Dear Sir/Madam Please let me know the difference between Chemiluminescent HRP Substrate (membrane) (ab5801) and ECL Western Blotting Substrate Kit (ab65623) because the customers do not know which one to choose for WB. Best regards.

    Thank you for your enquiry. I can confirm that both products (ab5801 and ab65623) can be used for Western blot application, however ab65623 can be applied for more test (50). Your customer may be interested in our very new range of WB reagents (Opt...

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    Do you have protein extraction kits?

    Thank you for calling Abcam. I have not been able to find a chemiluminescent EMSA kit in our catalogue. We do have several protein extraction kits though, please see the list below: Mammalian Cell Extraction Kit  ab65399 Plasma Membrane Protein Extract...

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    Thank you very much for reply. So do you supply the anti-pVIII protein of M13 phage antibody for ELISA (used in phage display experiment)? And HRP/AntiM13 and HRP substrate OPD (O-phenyldiamine dihydrochloride)?   Thank you very much.

    Thank you for contacting us. We have two anti-M13 antibodies that may be of interest: ab6188 Anti-M13 + Fd Bacteriophage Coat Proteins antibody Binds specifically to phage coat proteins of M13 or fd phage. ...

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    I am using your HMGB anitigen with antibody HMGB1 cat #ab12856 and your secondary ab6721 and getting a very low signal with traditional methods for HRP. I am look for a better signal but do not want to use film. Is the detection system ab5801 Ecl or...

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    I can confirm that the chemiluminescent peroxidase substrate ab5801 is actually a bit more sensitive than the ECL or ECL+ products. The fact you are having low signal with this substrate and the antibodies ab6721 and ab12856 could be due to a numbe...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"