• 产品名称
  • 描述
    兔多克隆抗体to CHD8
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 325-350 of Human CHD8 (NP_065971.2)

  • 阳性对照
    • HeLa cell lysate
  • 常规说明
    Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.


  • 形式
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituent: 99.91% Tris citrate/phosphate
    Note: pH: 7-8
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab114126 was affinity purified using an epitope specific to CHD8 immobilized on a solid support.
  • 克隆
  • 同种型
  • 研究领域


Our Abpromise guarantee covers the use of ab114126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 291 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • 功能
    DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating to efficient U6 RNA polymerase III transcription.
  • 序列相似性
    Belongs to the SNF2/RAD54 helicase family. CHD8 subfamily.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 翻译后修饰
  • 细胞定位
    Nucleus. Localizes to the promoter regions of several CTNNB1-responsive genes. Also present at known CTCF target sites.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent helicase CHD8 antibody
    • ATP-dependent helicase CHD8 antibody
    • Axis duplication inhibitor antibody
    • Beta catenin binding protein like antibody
    • CHD 8 antibody
    • CHD-8 antibody
    • chd8 antibody
    • CHD8_HUMAN antibody
    • Chromodomain helicase DNA binding protein 8 antibody
    • Chromodomain-helicase-DNA-binding protein 8 antibody
    • DKFZp686N17164 antibody
    • Duplin antibody
    • Helicase with SNF2 domain 1 antibody
    • HELSNF1 antibody
    • KIAA1564 antibody
    see all

Anti-CHD8 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CHD8 with ab114126 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-CHD8 antibody (ab114126) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 291 kDa

    Exposure time : 30 seconds
  • ab114126 at 3ug/mg of lysate detecting CHD8 by immunoprecipitation (lane 1). 1mg of HeLa whole cell lysate was used, 20% of immunoprecipitate was loaded. Lane 2: IgG control.
    Detection: chemiluminescence. Exposure time: 30 seconds.
  • All lanes : Anti-CHD8 antibody (ab114126) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK-1 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 291 kDa

    Exposure time : 3 minutes

Anti-CHD8 antibody (ab114126)参考文献

ab114126 has not yet been referenced specifically in any publications.

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I am sorry about the issues you are having with ab114126 in western blot. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples . If we cannot reso...

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