This fast track antibody is not yet fully characterized. It is subject to these terms and conditions




Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 22539951
IHC-P Use at an assay dependent concentration. PubMed: 24211491


  • 功能Probable transcription regulator.
  • 组织特异性Widely expressed in fetal and adult tissues.
  • 疾病相关Defects in CHD7 are a cause of CHARGE syndrome (CHARGES) [MIM:214800]. This syndrome, which is a common cause of congenital anomalies, is characterized by a non-random pattern of congenital anomalies including choanal atresia and malformations of the heart, inner ear, and retina.
    Genetic variations in CHD7 are associated with susceptibility to idiopathic scoliosis type 3 (IS3) [MIM:608765]. Idiopathic scoliosis (IS) is the most common spinal deformity in children.
    Defects in CHD7 are the cause of Kallmann syndrome type 5 (KAL5) [MIM:612370]. Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous.
    Defects in CHD7 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
  • 序列相似性Belongs to the SNF2/RAD54 helicase family.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP-dependent helicase CHD7 antibody
    • ATP-dependent helicase chromodomain helicase DNA binding protein 7 antibody
    • CHD-7 antibody
    • Chd7 antibody
    • CHD7_HUMAN antibody
    • Chromodomain helicase DNA binding protein 7 antibody
    • chromodomain helicase DNA binding protein 7 isoform CRA_e antibody
    • Chromodomain-helicase-DNA-binding protein 7 antibody
    • FLJ20357 antibody
    • FLJ20361 antibody
    • HH5 antibody
    • IS3 antibody
    • KAL5 antibody
    • KIAA1416 antibody
    see all

Anti-Chd7 antibody 图像

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • ab31824 at 1/500 staining mouse embryo tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue sections were formaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with the antibody for 20 minutes. An HRP conjugated donkey anti-rabbit antibody was used as the secondary.

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  • Immunohistochemical analysis of murine inner ear tissue, staining Chd7 with ab31824.

    Tissue was fixed in paraformaldehyde and blocked with 5% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/200) overnight at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody.

Anti-Chd7 antibody (ab31824)参考文献

This product has been referenced in:
  • Laurette P  et al. Transcription factor MITF and remodeller BRG1 define chromatin organisation at regulatory elements in melanoma cells. Elife 4:N/A (2015). WB ; Human . Read more (PubMed: 25803486) »
  • Tahara T  et al. Colorectal carcinomas with CpG island methylator phenotype 1 frequently contain mutations in chromatin regulators. Gastroenterology 146:530-538.e5 (2014). IHC-P ; Human . Read more (PubMed: 24211491) »

See all 14 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (BRAIN AND HEART)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (8)
Loading amount 30 µg
Specification BRAIN AND HEART
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5%

Abcam user community

Verified customer

提交于 Apr 25 2016

Thank you for your reply.

Although we do not have an antibody that is specifically guaranteed for WB of rat samples, I would like to recommend ab117522. The human and rat sequences share a high degree of homology at the N-terminus and the...

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Thank you for contacting Abcam regarding ab31824.

This antibody is a fast track antibody, meaning it has not been fully characterized. We have not tested this antibody in rat and cannot speak to its reactivity with this species based on l...

Read More

Thank you for your inquiry and I apologize for the delay. I heard back from our in-house Business Development team that this antibody is still considered "fast track," meaning that it is only guaranteed to work in ELISA against the immunizing pepti...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (HEK293, ES)
Loading amount 30 µg
Specification HEK293, ES
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Ms. Cindy Bartels

Verified customer

提交于 Oct 28 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Embryonic neural tube)
Specification Embryonic neural tube
Fixative Formaldehyde/acetic acid/EtOH
Antigen retrieval step None
Permeabilization Yes - 0.1% Tween 20
Blocking step Roche Blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C

Dr. Heather Etchevers

Verified customer

提交于 Apr 28 2008

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human embryonic kidney (Hek) 293 cells)
Specification human embryonic kidney (Hek) 293 cells
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

Dr. Erika Bosman

Verified customer

提交于 Mar 12 2008

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (embryo)
Specification embryo
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Ventana antibody diluent as blocking agent for 20 minute(s) · Concentration: undiluted

Dr. Erika Bosman

Verified customer

提交于 Feb 12 2007