Chimpanzee, Gorilla, Orangutan
Synthetic peptide, corresponding to a region between amino acids 2665- 2715 of Human CHD6 (NP_115597.3).
Whole cell lysate from Hela cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH: 7 to 8
Concentration information loading...
Immunogen affinity purified
ab114095 was affinity purified using an epitope specific to CHD6 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 305 kDa.
Use at 2-5 µg/mg of lysate.
Probable transcription regulator.
Belongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
ATP dependent helicase CHD6 antibody
ATP-dependent helicase CHD6 antibody
Western blot - Anti-CHD6 antibody (ab114095)
All lanes : Anti-CHD6 antibody (ab114095) at 0.1 µg/ml Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg Developed using the ECL technique. Predicted band size: 305 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-CHD6 antibody (ab114095)
ab114095, at 1 µg/ml, staining CHD6 in HeLa whole cell lysate immunoprecipitated using ab114095 at 3 µg/mg lysate (1 mg for IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 10 seconds.
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