Mouse, Rat, Horse, Chicken
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human CHD4.
This antibody gave a positive signal in HeLa (Human epithelial carcinoma cell line) nuclear lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 218 kDa (predicted molecular weight: 218 kDa).
Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
Belongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 2 PHD-type zinc fingers.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase.
Information by UniProt
ATP dependent helicase CHD4 antibody
ATP-dependent helicase CHD4 antibody
CHD 4 antibody
Western blot - CHD4 antibody (ab85620)
Anti-CHD4 antibody (ab85620) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
Additional bands at :
120 kDa,31 kDa. We are unsure as to the identity of these extra bands.
Exposure time :
has not yet been referenced specifically in any publications.
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