概述

  • 产品名称Anti-CHD4抗体
    参阅全部 CHD4 一抗
  • 描述
    兔多克隆抗体to CHD4
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human CHD4.

  • 阳性对照
    • This antibody gave a positive signal in HeLa (Human epithelial carcinoma cell line) Nuclear whole cell Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab85620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 218 kDa (predicted molecular weight: 218 kDa).

靶标

  • 功能Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
  • 序列相似性Belongs to the SNF2/RAD54 helicase family.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 PHD-type zinc fingers.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent helicase CHD4 antibody
    • ATP-dependent helicase CHD4 antibody
    • CHD 4 antibody
    • CHD-4 antibody
    • CHD4 antibody
    • CHD4_HUMAN antibody
    • Chromodomain helicase DNA binding protein 4 antibody
    • Chromodomain-helicase-DNA-binding protein 4 antibody
    • Mi 2 autoantigen 218 kDa protein antibody
    • Mi 2b antibody
    • Mi-2 autoantigen 218 kDa protein antibody
    • Mi2 beta antibody
    • Mi2-beta antibody
    see all

Anti-CHD4 antibody 图像

  • Anti-CHD4 antibody (ab85620) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 218 kDa
    Observed band size : 218 kDa
    Additional bands at : 120 kDa,31 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

Anti-CHD4 antibody (ab85620)参考文献

ab85620 has not yet been referenced specifically in any publications.

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