概述

  • 产品名称Anti-CHD4抗体
    参阅全部 CHD4 一抗
  • 描述
    小鼠单克隆抗体to CHD4
  • 经测试应用适用于: Flow Cyt, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: ETEPKGAADV EKVEEKSAID LTPIVVEDKE EKKEEEEKKE VMLQNGETPK DLNDEKQKKN IKQRFMFNIA DGGFTELHSL WQNEERAATV TKKTYEIWH, corresponding to amino acids 1632-1731 of Human CHD4

性能

应用

Our Abpromise guarantee covers the use of ab54603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 218 kDa.
IHC-P Use a concentration of 3 µg/ml.

靶标

  • 功能Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
  • 序列相似性Belongs to the SNF2/RAD54 helicase family.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 PHD-type zinc fingers.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent helicase CHD4 antibody
    • ATP-dependent helicase CHD4 antibody
    • CHD 4 antibody
    • CHD-4 antibody
    • CHD4 antibody
    • CHD4_HUMAN antibody
    • Chromodomain helicase DNA binding protein 4 antibody
    • Chromodomain-helicase-DNA-binding protein 4 antibody
    • Mi 2 autoantigen 218 kDa protein antibody
    • Mi 2b antibody
    • Mi-2 autoantigen 218 kDa protein antibody
    • Mi2 beta antibody
    • Mi2-beta antibody
    see all

Anti-CHD4 antibody 图像

  • CHD4 antibody (ab54603) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human transitional cell carcinoma.


  • Predicted band size : 218 kDa
    CHD4 antibody (ab54603) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab54603 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54603, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was a mix of mouse IgG2a [ICIGG2A], (ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-CHD4 antibody (ab54603)参考文献

This product has been referenced in:
  • Trakarnsanga K  et al. Induction of adult levels of ß-globin in human erythroid cells that intrinsically express embryonic or fetal globin by transduction with KLF1 and BCL11A-XL. Haematologica 99:1677-85 (2014). WB . Read more (PubMed: 25107887) »
  • Sparmann A  et al. The chromodomain helicase Chd4 is required for Polycomb-mediated inhibition of astroglial differentiation. EMBO J 32:1598-612 (2013). IHC-P . Read more (PubMed: 23624931) »

See all 5 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - other (U2OS cells)
Gel Running Conditions Reduced Denaturing (6%)
Loading amount 10 µg
Specification U2OS cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 May 11 2015

Application Immunoprecipitation
Immuno-precipitation step Protein A
Sample Human Cell lysate - nuclear (U2OS)
Specification U2OS
Treatment 500nM Cisplatin
Total protein in input 500 µg
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提交于 Nov 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"