概述
-
产品名称Anti-CHD3抗体[EPNCIR110A]
参阅全部 CHD3 一抗 -
描述兔单克隆抗体[EPNCIR110A] to CHD3
-
宿主Rabbit
-
经测试应用适用于: Flow Cyt, WB, IHC-P, ICC/IFmore details
不适用于: IP -
种属反应性与反应: Mouse, Rat, Human
-
免疫原
Synthetic peptide within Mouse CHD3 aa 1-100. The exact sequence is proprietary.
Database link: B1AR17 -
阳性对照
- K562, HeLa, C6, and PC12 cell lysates, Human breast and Human ovary tissues; HeLa cells
-
常规说明
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager. View antibodies from NCI Center for Cancer Research Collaboration
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
性能
-
形式Liquid
-
存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
-
存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
-
Concentration information loading... -
纯度Protein A purified
-
克隆单克隆
-
克隆编号EPNCIR110A
-
同种型IgG
-
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Positive Controls
应用
Our Abpromise guarantee covers the use of ab109195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 260 kDa (predicted molecular weight: 227 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes) | |
| ICC/IF | 1/100 - 1/250. |
靶标
-
功能Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
-
组织特异性Widely expressed.
-
序列相似性Belongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 PHD-type zinc fingers. -
细胞定位Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase and mitosis.
- Information by UniProt
-
数据库链接
- Entrez Gene: 1107 Human
- Entrez Gene: 216848 Mouse
- Entrez Gene: 303241 Rat
- Omim: 602120 Human
- SwissProt: Q12873 Human
- Unigene: 25601 Human
- Unigene: 178246 Mouse
- Unigene: 234402 Rat
-
别名
- 226 antibody
- 2600010P09Rik antibody
- AF020312 antibody
see all
图片
-
![Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)](http://www.abcam.cn/ps/products/109/ab109195/Images/ab109195-256171-ab109195KOWB.jpg)
Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CHD3 knockout HAP1 cell lysate (20 µg)
Lane 3: Raji cell lysate (20 µg)
Lane 4: Y79 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109195 observed at 245 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109195 was shown to specifically react with CHD3 when CHD3 knockout samples were used. Wild-type and CHD3 knockout samples were subjected to SDS-PAGE. ab109195 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW)preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)](http://www.abcam.cn/ps/products/109/ab109195/Images/CHD3-Primary-antibodies-ab109195-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)Immunohistochemical staining of paraffin-embedded Human breast tissue using ab109195 at a dilution of 1/100. -
![Flow Cytometry - Anti-CHD3 antibody [EPNCIR110A] (ab109195)](http://www.abcam.cn/ps/products/109/ab109195/Images/ab109195-276947-ab109195FC1.jpg)
Flow Cytometry - Anti-CHD3 antibody [EPNCIR110A] (ab109195)Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CHD3 with purified ab109195 at 1/230 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
-
![Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)](http://www.abcam.cn/ps/products/109/ab109195/Images/CHD3-Primary-antibodies-ab109195-5.JPG)
Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)All lanes : Anti-CHD3 antibody [EPNCIR110A] (ab109195) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : C6 cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 227 kDa
Observed band size: 260 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)](http://www.abcam.cn/ps/products/109/ab109195/Images/CHD3-Primary-antibodies-ab109195-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)Immunohistochemical staining of paraffin-embedded Human ovary tissue using ab109195 at a dilution of 1/100. -
![Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] (ab109195)](http://www.abcam.cn/ps/products/109/ab109195/Images/CHD3-Primary-antibodies-ab109195-4.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] (ab109195)Immunofluorescent staining of HeLa cells using ab109195 at a dilution of 1/100.
实验方案
文献
ab109195 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"