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Synthetic peptide within Mouse CHD3 aa 1-100. The exact sequence is proprietary.
Database link: B1AR17
This product is a recombinant rabbit monoclonal antibody.
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager. View antibodies from NCI Center for Cancer Research Collaboration
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Our Abpromise guarantee covers the use of ab109195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 260 kDa (predicted molecular weight: 227 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)|
|ICC/IF||1/100 - 1/250.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CHD3 knockout HAP1 cell lysate (20 µg)
Lane 3: Raji cell lysate (20 µg)
Lane 4: Y79 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109195 observed at 245 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109195 was shown to specifically react with CHD3 when CHD3 knockout samples were used. Wild-type and CHD3 knockout samples were subjected to SDS-PAGE. ab109195 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
ab109195 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"