概述

  • 产品名称Anti-CHD3抗体
    参阅全部 CHD3 一抗
  • 描述
    兔多克隆抗体to CHD3
  • 经测试应用适用于: IP, IHC-Pmore details
    不适用于: WB
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 1950-2000 of Human CHD3 (NP_001005273.1).

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab114100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
    • 组织特异性Widely expressed.
    • 序列相似性Belongs to the SNF2/RAD54 helicase family.
      Contains 2 chromo domains.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 2 PHD-type zinc fingers.
    • 细胞定位Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase and mitosis.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 226 antibody
      • 2600010P09Rik antibody
      • AF020312 antibody
      • ATP dependent helicase CHD3 antibody
      • ATP-dependent helicase Chd3 antibody
      • CHD-3 antibody
      • Chd3 antibody
      • CHD3_HUMAN antibody
      • Chd7 antibody
      • Chromodomain-helicase-DNA-binding protein 3 antibody
      • hZFH antibody
      • MGC40857 antibody
      • Mi 2 autoantigen 240 kDa protein antibody
      • Mi 2a antibody
      • Mi-2 autoantigen 240 kDa protein antibody
      • Mi2 ALPHA antibody
      • Mi2-alpha antibody
      • Prp7 antibody
      • Prp9 1 antibody
      • ZFH antibody
      • Zinc finger helicase antibody
      • zinc-finger helicase (Snf2-like) antibody
      see all

    Anti-CHD3 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ewing sarcoma (left) and mouse renal cell carcinoma (right) tissues labelling CHD3 with ab114100 at 1/1000 (1µg/ml). Detection: DAB.
    • Rabbit anti-CHD3 antibody, at 1 µg/ml, staining CHD3 in HeLa whole cell lysate immunoprecipitated using ab114100 at 3 µg/mg lysate (1 mg for IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 10 seconds.

    Anti-CHD3 antibody (ab114100)参考文献

    ab114100 has not yet been referenced specifically in any publications.

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