The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2.5 µg/ml. Predicted molecular weight: 101 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能DNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding.
组织特异性Frequently overexpressed in hepatomacellular carcinomas.
序列相似性Belongs to the SNF2/RAD54 helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 Macro domain.
结构域The macro domain mediates non-covalent poly(ADP-ribose)-binding and recruitment to DNA damage sites.
细胞定位Nucleus. Localizes at sites of DNA damage. Probably recruited to DNA damage sites by PARylated PARP1.